For calibration curve samples, human AC16 cells (Millipore) were cultured in DMEM/F12 supplemented with 10% FBS and either 6% D2O (heavy labeled population) or 6% H2O (control population) at 37°C, 5% CO2. The cells were maintained in this medium for 3 passages, each passage with a split ratio of 1:8...
ORGANISM(S): Homo Sapiens (human)
