Parental HeLa cells and HeLa cells stably expressing PINK1-3FLAG were treated with or without 10 μM CCCP for 3 h, incubated with 0.1% paraformaldehyde for 10 min at room temperature, and then quenched with 100 mM glycine-NaOH (pH7.5) for 4 min. After washing twice with 20 mM HEPES-NaOH (pH7.4) and 1...
ORGANISM(S): Homo Sapiens (human)