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To quantify TIM23 and PINK1-3HA in the immunoprecipitates, control or PINK1-3HA expressing HeLa cells were treated with CCCP and subjected to immunoprecipitation with anti-HA antibody bound to magnetic beads. After spiked with 200 fmol stable isotope-labeled AQUA peptides (Cosmo Bio Co Ltd) as stand...
ORGANISM(S): Homo Sapiens (human) 
Parental HeLa cells and HeLa cells stably expressing PINK1-3FLAG were treated with or without 10 μM CCCP for 3 h, incubated with 0.1% paraformaldehyde for 10 min at room temperature, and then quenched with 100 mM glycine-NaOH (pH7.5) for 4 min. After washing twice with 20 mM HEPES-NaOH (pH7.4) and 1...
ORGANISM(S): Homo Sapiens (human) 
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