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High-throughput optimized prime editing-mediated endogenous protein tagging for pooled imaging of protein localization
The subcellular organization of proteins carries important information on cellular state and gene function, yet currently there is a limitation in technologies that enable routine measurement of protein localizations at scale. Here we develop pooled endogenous protein tagging using prime editing to ...
ORGANISM(S): Homo sapiens 
2026-09-20 | GSE346707 | GEO
Objective DLL1 is a mammalian Notch ligand with essential functions during embryonic development. We tagged endogenous DLL1 in one homologous recombination step such that AcGFP-HA tagged DLL1 could be converted by Cre-mediated site-specific recombination into StrepFlag tagged DLL1. We anticipated th...
ORGANISM(S): Mus musculus (Mouse) 
2021-11-03 | PXD024680 | Pride
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