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TranscriptomicsTranscription profiling of E. coli O157:H7 growth in the absence or presence of HEp-2 epithelial cells
E. coli O157:H7 global gene expression profile is altered during growth in the presence of Hep-2 cells, relative to the absence of cells; We used Affymetrix E. coli 2.0 genechips to determine gene expression changes Experiment Overall Design: E. coli O157:H7 total RNA extracts were collected after a...
ORGANISM(S): Escherichia coli O157:H7 
2009-03-28 | E-GEOD-14238 | biostudies-arrayexpress
TranscriptomicsEnterohemorrhagic Escherichia coli (EHEC)8624 double deletions of qseC and qseE grown in DMEM
Analysis of effect of deletion of the two adrenergic kinases QseC and QseE on gene expression of EHEC 8624 in the absence and presence of epinephrine A double mutant of qseC and qseE in the EHEC 8624 was grown in the absence or presence of epinephrine to OD600=1.0 in DMEM then processed according to...
ORGANISM(S): Escherichia coli O157:H7 
2012-03-15 | E-GEOD-33895 | biostudies-arrayexpress
TranscriptomicsThe Apple Flavonoid Phloretin Inhibits Escherichia coli O157:H7 Biofilm Formation and Ameliorates Colon Inflammation in Rat
Pathogenic biofilms have been associated with persistent infections due to high resistance to antimicrobial agents while commensal biofilms often fortify host immune system. Hence, controlling biofilm formation of both pathogenic bacteria and commensal bacteria is important in bacteria-related dise...
ORGANISM(S): Escherichia coli 
2011-11-22 | E-GEOD-28144 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli to investigate bacterial adrenergic signaling
The ability to respond to stress is at the core of an organism’s survival. The hormones epinephrine and norepinephrine play a central role in stress responses in mammals, which require the synchronized interaction of the whole neuroendocrine system. Bacteria also sense and respond to epinephrine a...
ORGANISM(S): Escherichia coli 
2009-11-16 | E-GEOD-15050 | biostudies-arrayexpress
TranscriptomicsEthanolamine gene regulation in EHEC
Bacterial pathogens must be able to both recognize suitable niches within the host for colonization and successfully compete with commensal flora for nutrients in order to establish infection. Ethanolamine (EA) is a major component of mammalian and bacterial membranes and may be used by pathogens as...
ORGANISM(S): Escherichia coli 
2012-06-17 | E-GEOD-34046 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Escherichia coli strain 8624 and Escherichia coli strain VS94 grown with various signaling molecules
These E. coli strains were grown with various signaling molecules and the expression profiles were determined. Experiment Overall Design: These E. coli strains were grown with various signaling molecules and the expression profiles were determined using the Affymetrix E. coli 2.0 Array. Experiment O...
ORGANISM(S): Escherichia coli 
2008-06-15 | E-GEOD-7439 | biostudies-arrayexpress
TranscriptomicsEnterohemorrhagic Escherichia coli (EHEC) 8624 double deletions of kdpe and cra grown in DMEM
Analysis of effect of deletion of the two transcriptional factors KdpE and Cra on gene expression of EHEC 8624 A double mutant of kdpE and cra in the EHEC 8624 was grown to OD600=1.0 in DMEM then processed according to manufacturer's specifications: http//www.affymetrix.com/support/technical/manual...
ORGANISM(S): Escherichia coli 8624 
2013-07-03 | E-GEOD-43659 | biostudies-arrayexpress
TranscriptomicsGenomic Comparison of the O-antigen Biosynthesis Gene Clusters of E. coli O55 Strains Belonging to Three Lineages.
Typical enteropathogenic Escherichia coli (EPEC) O55:H7 is regarded as the closest relative of enterohemorrhagic E. coli (EHEC) O157:H7. Both serotypes usually express the γ1 intimin subclass and trigger actin polymerazation by the Tir-TccP pathway. However, atypical O55:H7 strains capable of trigg...
ORGANISM(S): Escherichia coli 
2010-05-22 | E-GEOD-8889 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli RCV02 (cadA deficient) v wild-type enterohemorrhagic E. coli (EHEC)
Adherence of pathogenic Escherichia coli strains to intestinal epithelia is essential for infection. For enterohemorrhagic E. coli (EHEC) serotype O157:H7, we have previously demonstrated that multiple factors govern this pathogen’s adherence to HeLa cells (39). One of these factors is CadA, a lys...
ORGANISM(S): Escherichia coli 
2008-10-25 | E-GEOD-11927 | biostudies-arrayexpress
TranscriptomicsBile Salts Influence Virulence Properties of E. coli O157:H7
Enterohemorrhagic Escherichia coli (EHEC), including serotype O157:H7, cause severe food-borne illness. On route to the human colon, they encounter and resist, numerous anti-microbial ingestion stresses. We hypothesize that these stresses cue EHEC to alter virulence properties. This study investigat...
ORGANISM(S): Escherichia coli 
2011-09-05 | E-GEOD-22060 | biostudies-arrayexpress
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