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GenomicsChIP-Seq of ERalpha and RNA polymerase II defines genes differentially responding to ligands
We used ChIP-Seq to map ERalpha binding sites and to profile changes in RNA polymerase II (RNAPII) occupancy in MCF-7 cells in response to estradiol (E2), tamoxifen or fulvestrant. We identified 10,205 high confidence ERalpha binding sites in response to E2 of which 68% contained an estrogen respons...
ORGANISM(S): Homo sapiens 
2009-03-09 | E-GEOD-14664 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human MDA-MB-231 cells response to adenovirus expressed estradiol-ERalpha, estradiol-Erbeta, and ERE Binding defective mutants
This SuperSeries is composed of the following subset Series:; GSE9757: Response to estradiol-ERalpha; GSE9758: Response to estradiol-ERalpha ERE Binding defective mutant; GSE9759: Response to estradiol-Erbeta and estradiol-ERbeta ERE binding defective mutant Experiment Overall Design: Refer to indiv...
ORGANISM(S): Homo sapiens 
2008-06-16 | E-GEOD-9761 | biostudies-arrayexpress
TranscriptomicsGlobal analysis of ZNF217 chromatin occupancy in the breast cancer cell genome reveals an association with Eralpha
Background: The ZNF217 gene, encoding a C2H2 zinc finger protein, is located at 20q13 and found amplified and overexpressed in greater than 20% of breast tumors. Current studies indicate ZNF217 drives tumorigenesis, yet the regulatory mechanisms of ZNF217 are largely unknown. Because ZNF217 associat...
ORGANISM(S): Homo sapiens 
2014-06-15 | E-GEOD-58326 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human breast cancer cells expressing ERalpha and treated with 17beta-estradiol
Gene expression changes caused by estrogen treatment of breast cancer cells that re-express ERalpha was investigated by infecting ER-negative MDA-MB-231 breast cancer cells for 24 h with recombinant adenovirus encoding full-length human ERalpha (Ad-ERalpha) or control vector (Ad-LacZ), and treating ...
ORGANISM(S): Homo sapiens 
2007-08-25 | E-GEOD-2251 | biostudies-arrayexpress
GenomicsGenome-wide analysis of ERalpha binding sites in U2OS-ERalpha cells
ChIP followed by deep sequencing was performed with antibodies to ERalpha in U2OS-ERalpha cells treated with 17beta-estradiol. Examination of Eralpha binding sites in U2OS-Eralpha cells. Sequenced input was used as a control
ORGANISM(S): Homo sapiens 
2011-05-03 | E-GEOD-28918 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human cell response to estradiol-ERalpha
In addition to the estrogen responsive element (ERE)-dependent gene expression, E2-ERalpha regulates transcription through functional interactions with transfactors bound to their cognate regulatory elements on DNA, hence the ERE-independent signaling pathway. However, the relative importance of th...
ORGANISM(S): Homo sapiens 
2008-06-16 | E-GEOD-9757 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human cancer cell line MCF-7 - estrogen effects where MCF7 is co-expressing ERa and ERb
Two subtypes of the estrogen receptor, ERalpha and ERbeta, mediate the actions of estrogens, and the majority of human breast tumors contain both ERalpha and ERbeta. To examine the possible interactions and modulatory effects of ERbeta on ERalpha activity, we have used adenoviral gene delivery to pr...
ORGANISM(S): Homo sapiens 
2009-04-24 | E-GEOD-4006 | biostudies-arrayexpress
TranscriptomicsERalpha ChIP-chip from HeLA-ER cells treated with E2
We are studying the mechanisms by which the estrogen receptor, ERalpha, is recruited to and regulates genes with a non-direct DNA binding. We performed ChIP-chip for ERalpha in E2 treated HeLa-ER cells, and looked at 19000 RefSeq genes to determine binding patterns of the receptor at promoters. Th...
ORGANISM(S): Homo sapiens 
2011-02-22 | E-GEOD-22099 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human cells transfected with the recombinant adenovirus bearing no cDNA (CMV) or cDNA for ERalpha 203/204/211E (3411E) for 48h
In addition to the estrogen responsive element (ERE)-dependent gene expression, E2-ERalpha regulates transcription through functional interactions with transfactors bound to their cognate regulatory elements on DNA, hence the ERE-independent signaling pathway. However, the relative importance of th...
ORGANISM(S): Homo sapiens 
2008-06-16 | E-GEOD-9758 | biostudies-arrayexpress
GenomicsChIP-Seq of human MCF-7 cells with ER-alpha anitbody following estrogen treatment
We performed chromatin immunoprecipitation (ChIP) with two polyclonal anti-ERalpha antibodies directed against the N- and C-terminus of the protein, with or without estrogen treatment for 45 minutes of MCF-7 cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer...
ORGANISM(S): Homo sapiens 
2010-01-11 | E-MTAB-131 | biostudies-arrayexpress
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