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GenomicsTET1 is a maintenance DNA demethylase that prevents methylation spreading in adult cells [MeDIP-Seq]
We report that full length TET1 (TET1-FL) overexpression fails to induce global DNA demethylation in HEK293T cells. The preferential binding of TET1-FL to hypomethylated CpG islands (CGIs) through its CXXC domain leads to its inhibited 5-hydroxymethylcytosine (5hmC) production as methylation level i...
ORGANISM(S): Homo sapiens 
2014-06-04 | E-GEOD-44036 | biostudies-arrayexpress
TranscriptomicsTET1 is a maintenance DNA demethylase that prevents methylation spreading in adult cells [RNA-seq]
We report that full length TET1 (TET1-FL) overexpression fails to induce global DNA demethylation in HEK293T cells. The preferential binding of TET1-FL to hypomethylated CpG islands (CGIs) through its CXXC domain leads to its inhibited 5-hydroxymethylcytosine (5hmC) production as methylation level i...
ORGANISM(S): Homo sapiens 
2014-06-04 | E-GEOD-49833 | biostudies-arrayexpress
TranscriptomicsTET1 is a maintenance DNA demethylase that prevents methylation spreading in adult cells [cDNA microarray]
We report that full length TET1 (TET1-FL) overexpression fails to induce global DNA demethylation in HEK293T cells. The preferential binding of TET1-FL to hypomethylated CpG islands (CGIs) through its CXXC domain leads to its inhibited 5-hydroxymethylcytosine (5hmC) production as methylation level i...
ORGANISM(S): Homo sapiens 
2014-06-04 | E-GEOD-50016 | biostudies-arrayexpress
GenomicsConserved DNA methylation patterns in healthy blood cells and extensive changes in leukemia measured by a new quantitative technique.
We describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on sequential DNA digestion with a pair of methylation-blocked and methylation-tolerant neoschizomeric restriction enzymes SmaI/XmaI followed by end repair and ultra-deep sequencing. DREAM provides informa...
ORGANISM(S): Homo sapiens 
2012-11-08 | E-GEOD-39787 | biostudies-arrayexpress
TranscriptomicsDNA methylation analysis of Colon Cancer cell line SW48
We combined gene expression experiments in response to HDAC inhibitor Depsipeptide with DNA methylation level to assess gene reactivation arising from hypermethylated promoters. We did one experiment with 1 untreated and 1 treated batch of cells
ORGANISM(S): Homo sapiens 
2012-04-03 | E-GEOD-34427 | biostudies-arrayexpress
TranscriptomicsColon Cancer cell line YB5 untreated cells vs YB5 treated 24h with Depsipeptide at 20 nM
Study the effect of HDACi Depsipeptide on gene expression. We combined these data with whole genome DNA methylation microarray to test the reactivation of genes that have an hypermethylated promoter region. We did two independant experiments with 2 untreated and 2 treated different batch of cells
ORGANISM(S): Homo sapiens 
2012-04-03 | E-GEOD-34077 | biostudies-arrayexpress
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