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MetabolomicsCharacterization of Whole Blood EVs Using PRotein Organic Solvent PRecipitation

Blood plays a vital role in diagnostic medicine by providing essential information for assessing health and diagnosing various conditions. Routine blood tests can reveal important biomarkers that support early diagnosis and timely intervention. Recently, extracellular vesicles (EVs) have emerged ...

2026-08-24 | MTBLS12801 | MetaboLights
MetabolomicsBacteria regulate aging physiology in microalgae via induced vesicle production and the methionine cycle (Metabolomics profiling of FACS purified EVs)

In annually reoccurring patterns, microalgae form blooms that persist and decline thereby contributing massively to global biogeochemical cycles. The decline of blooms is mainly caused by nutrient limitation and goes ahead with the aging of individual algal cells. Nutrient intake can re-initiate ...

2024-05-07 | MTBLS5401 | MetaboLights
GenomicsComparison of EVs isolation methods for miRNAs sequencing
Comparison of EVs isolation methods for miRNAs sequencing
PRJNA928492 | ENA
ProteomicsIsolation and Characterisation of EVs from Feline Plasma: A Comparative Analysis with Human Plasma Derived EVs
Extracellular vesicles (EVs) are nanoparticles found in all biological fluids, capable of transporting biological material around the body. Extensive research into the physiological role of EVs has led to the development of the Minimal Information for Studies of Extracellular Vesicles (MISEV) framew...
ORGANISM(S): Homo sapiens (Human) 
2022-07-13 | PXD034369 | Pride
Human Extracellular vesicles Cat Bruker timstof pro 
TranscriptomicsFluidigm Biomark 96.96 nanofluidic arrays for qRT-PCR of 93 miRNAs: salivary extracellular vesicles
Crude extracellular vesicles (EVs) from eight healthy volunteers were separated into 6 fractions based on their densities by using the iodixanol-based density gradient centrifugation method. To determine the distribution of miRNAs among these fractions, quantities of 93 miRNAs were quantified by the...
ORGANISM(S): Homo sapiens 
2016-08-01 | E-GEOD-78763 | biostudies-arrayexpress
GenomicsComparison of EVs isolation methods for miRNAs sequencing
Background & aims: MicroRNAs (miRNAs) encapsulated in EVs are potential diagnostic and prognostic biomarkers. However, discrepancies on miRNA patterns and their validation are still frequent due to differences in sample origin, EVs isolation, miRNA extraction and sequencing methods. Selecting approp...
ORGANISM(S): Homo sapiens 
2023-04-26 | GSE223838 | GEO
TranscriptomicsRNA-Seq of effects of exercise-induced Extracellular Vesicles on an F344 rat (Rattus norvegicus) model of forced running wheel exercise
n total, 16 7-week-old male F344 rats were subjected to model regular exercise and sedentary lifestyle. In the second week after arrival and acclimatization to the reversed light/dark cycle, rats were introduced to the forced running wheel without a specific running mode. It was followed by a 12-day...
ORGANISM(S): Rattus norvegicus 
2021-12-28 | E-MTAB-11020 | biostudies-arrayexpress
TranscriptomicsRapid isolation of extracellular vesicles from cell culture and biological fluids using a synthetic peptide with specific affinity for heat shock proteins
Background: Exosomes and extracellular vesicles (EVs) are increasingly recognized as important sources of biomarkers for disease study and diagnosis. Results: A synthetic peptide, Vn96, allows for capture of EVs from biological fluids using basic laboratory equipment. Conclusion: The Vn96-captured E...
ORGANISM(S): Homo sapiens 
2014-06-14 | E-GEOD-58464 | biostudies-arrayexpress
TranscriptomicsmicroRNA expression profile in isolated human pancreatic islets and in human pancreatic islet-derived extracellular vesicles -EVs-
Human pancreatic islets were isolated from pancreas of deceased donors by Ricordi's procedure and cultured in CMRL 1066 medium additioned with human albumin. EVs were isolated from conditioned medium derived from islet culture after isolation. Once isolated, RNA of islets and islet-derived EVs was e...
ORGANISM(S): Homo sapiens 
2012-07-30 | E-MEXP-3691 | biostudies-arrayexpress
TranscriptomicsComparative transcriptomic analysis of human and Drosophila extracellular vesicles reveals extensive conservation
Extracellular vesicles (EVs) are membrane-enclosed nanoparticles containing specific repertoires of genetic material. In mammals, EVs can mediate the horizontal transfer of various cargos and signaling molecules, notably miRNA and mRNA species. Whether this form of intercellular communication prevai...
ORGANISM(S): Homo sapiens 
2016-06-15 | E-GEOD-76173 | biostudies-arrayexpress
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