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Transcriptome comparison of two isogenic strains, one with a wild type cyoB allele and another with an inactivated cyoB allele
ORGANISM(S): Pseudomonas putida 
Overnight cultures of wild type COL or the bshA mutant were back-diluted to an O.D.650 of 0.1 in 30 mL of TSB. The cells were grown at 37C with shaking in a 300mL flask until they reached an O.D.650 of 1.1. The cells were then equally separated into 50mL Falcon tubes, so that each tube had 10mL of c...
ORGANISM(S): Staphylococcus aureus subsp. aureus COL 
Bladder cancer is one of the most common cancers. Since prognosis ameliorates with early detection, it is a challenge to develop techniques that could replace or complement the current diagnosis protocols. The study of extracellular vesicles (EVs) that are present in urine samples has become an attr...
ORGANISM(S): Homo sapiens 
Comparison of genomic DNA from two strains of Xylella fastidiosa: 9a5c (pathogenic, reference strain) and J1a12 (non-pathogenic).
ORGANISM(S): Xylella fastidiosa 

Background

Cancer metabolism is emerging as an important focus area in cancer research. However, the in vitro cell culture conditions under which much cellular metabolism research is performed differ drastically from in vivo tumor conditions, which are characterized by variations in the leve...
2021-11-05 | MTBLS150 | MetaboLights
Recently, multicellular spheroids were isolated from a well-established epithelial ovarian cancer cell line, OVCAR-3, and were propagated in vitro. These spheroid derived cells displayed numerous hallmarks of cancer stem cells, which are chemo and radioresistant cells thought to be a significant cau...
2015-01-08 | MTBLS152 | MetaboLights

Transcriptomic studies attempted to classify glioblastoma (GB) into subtypes that predict survival and that have different therapeutic vulnerabilities1-3. Here we identified three metabolic subtypes: glycolytic, oxidative and a mixed glycolytic/oxidative by mass spectrometry imaging of rapidly ex...

2025-05-08 | MTBLS12176 | MetaboLights
The decline in adaptive immunity, naïve T-cell output and a contraction in the peripheral T cell receptor (TCR) repertoire with age are largely attributable to thymic involution and the loss of critical cytokines and hormones within the thymic microenvironment. To assess the molecular changes associ...
ORGANISM(S): Mus musculus 
The success of targeted therapies creates a need to discriminate tumors accurately by their histological and genetic characteristics. Here, we used cDNA microarray analysis to identify gene expression profiles and single markers that recapitulate the pathological and genetic background (i.e., BRAF, ...
ORGANISM(S): Homo sapiens 
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