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TranscriptomicsChromatin immunoprecipitation of mouse tumour cell line L1210 synchronized and sampled after mitosis to map replication time zones in the mouse genome
Measuring replication time of regions in teh mouse genome, using a novel synchronization method, labeling of BrdU, immunoprecipitation and hybridization to microarrays
ORGANISM(S): Mus musculus 
2008-08-04 | E-MEXP-1022 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse lymphoblast cell line L1210 to validate replication timing experiments
In this experiment, total RNA was extracted from asynchronous population of L1210 cells and hybridized to Affymetrix 430A 2.0 arrays in order to obtain an expression profile of these cells. We have previously mapped the replication timing of the entire mouse genome in this cell line, using mouse CGH...
ORGANISM(S): Mus musculus 
2008-08-04 | E-MEXP-1681 | biostudies-arrayexpress
TranscriptomicsHuman Cells Time of Replication (ToR)
Measurment of the ToR by comapring the DNA content of the S phase and the DNA content of the G1 phase, the method is based on the method used in the paper - Replication timing of the human genome by Woodfine et al. that was published in Hum. Mol. Genet. 2004. Briefly, after staining with Propidium I...
ORGANISM(S): Homo sapiens 
2010-05-24 | E-GEOD-17235 | biostudies-arrayexpress
TranscriptomicsMethylation profiling in various human cell lines and tissues by mDIP - methylated DNA precipitation with antibodies against methylated cytosine
Finding the methylation state of DNA promoters in various cell lines and tissues by mDIP - methylated DNA precipitation with antibodies against methylated cytosine.
ORGANISM(S): Homo sapiens 
2006-02-01 | E-MEXP-520 | biostudies-arrayexpress
TranscriptomicsMouse Cells Time of Replication (ToR)
Measurment of the ToR by comapring the DNA content of the S phase and the DNA content of the G1 phase, the method is based on the method used in the paper - Replication timing of the human genome by Woodfine et al. that was published in Hum. Mol. Genet. 2004. Briefly, after staining with Propidium I...
ORGANISM(S): Mus musculus 
2010-05-25 | E-GEOD-17229 | biostudies-arrayexpress
TranscriptomicsChromatin immunopreciptiation of human BJ-T cells with E2F2 and EwF4 antibodies
ChIp on chip with E2F2 or E2F4 antibodies on BJ-T cells.
ORGANISM(S): Homo sapiens 
2009-11-16 | E-MEXP-2169 | biostudies-arrayexpress
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