Fedbatch cultivation

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2021 (2)
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Transcription profiling by array (1)

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2012 (1)

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Cell Culture Technology, Technical Faculty, Bielefeld University, Germany (2)

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Time-resolved proteomic profiling for CHO-K1 fed-batch cultivations

The majority of recombinant protein therapeutics are produced with Chinese Hamster Ovary (CHO) cells. Productivity depends on the initial cell line engineering in terms of integration site or choice of an appropriate promotor for the recombinant gene expression, as well as media and process paramete...

ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

2021-01-25 | PXD018439 | Pride

Influence of oxygen limitation, absence of the cytochrome bc1 complex and low pH on global gene expression in Gluconobacter oxydans 621H using DNA microarray technology

The genome-wide transcriptional responses of the strictly aerobic -proteobacterium Gluconobacter oxydans 621H to oxygen limitation, to the absence of the cytochrome bc1 complex, and to low pH were studied using DNA microarray analyses. Oxygen limitation caused expression changes of 486 genes, repres...

ORGANISM(S): Gluconobacter oxydans 621H

2012-03-23 | E-GEOD-34294 | biostudies-arrayexpress

TECAN Resolvex A200 Positive Pressure Workstation for Semi Automated Peptide Purification of Complex Proteomic Samples

High-throughput has become a substantial need in many omics investigations. In proteomics the sample preparation workflow consists of multiple steps, whereby with each additional manual step more bias is added to the sample. Especially for label-free quantification experiments this drastically reduc...