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ProteomicsIntegrated bottom-up and top-down proteomics of patient-derived breast tumor xenografts

Ntai, I., LeDuc, R.D., Fellers, R.T., et al., (2015) Molecular and Cellular Proteomics, Manuscript M114.047480

Bottom-up proteomics relies on the use of proteases and is the method ...

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2019-08-28 | MSV000084247 | MassIVE
ProteomicsBioinformatics Analysis of Top-Down Mass Spectrometry Data with ProSight Lite
Datafiles for Bioinformatics Analysis of Top-Down Mass Spectrometry Data with ProSight Lite
ORGANISM(S): Equus Caballus (ncbitaxon:9796) Bos Taurus (ncbitaxon:9913) 
2021-04-13 | MSV000087182 | MassIVE
ProteomicsUnique Histone Proteoforms by Differential Top Down MS
H3.1 and H3.3 quadrupole isolated and fragmented by ETD and EThcD on an Orbitrap Fusion Lumos. Comparison of two cell lines.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-07-14 | MSV000079191 | MassIVE
ProteomicsOrbitrap mass spectrometry and high-field asymmetric waveform ion mobility spectrometry (FAIMS) enable the indepth analysis of human serum proteoforms
In this study, we describe a straightforward protocol for intact proteoform sample preparation based on depletion of albumin and immunoglobulins followed by simplified fractionation of remaining serum proteins via polyacrylamide gel electrophoresis. After molecular weight-based fractionation, we sup...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-02 | MSV000092574 | MassIVE
ProteomicsOrbitrap mass spectrometry and high-field asymmetric waveform ion mobility spectrometry (FAIMS) enable the in-depth analysis of human serum proteoforms
After molecular weight-based fractionation, we supplemented the traditional liquid chromatography tandem mass spectrometry (LC-MS2) data acquisition with high-field asymmetric waveform ion mobility spectrometry (FAIMS), which served as an additional separation dimension to further simplify serum pro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-02-16 | MSV000091309 | MassIVE
ProteomicsTargeted Quantification of Proteoforms in Human Samples by Proteoform Reaction Monitoring
Here, we describe an approach based on the principle of PRM for the measurement of intact proteoforms by targeted top-down proteomics termed Proteoform Reaction Monitoring (PfRM). We explore the ability of our method to circumvent traditional limitations of top-down proteomics such as sensitivity an...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-21 | MSV000091774 | MassIVE
ProteomicsSingle Cell Proteoform Analysis Using scPiMS
We introduce single-cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells drop casted onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enables...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-02-02 | MSV000093994 | MassIVE
ProteomicsNext-generation Serology by Mass Spectrometry:Readout of the SARS-CoV-2 Antibody Repertoire
Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses new advances i...
ORGANISM(S): Homo Sapiens 
2022-09-24 | PXD028627 | panorama
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