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Transformed AC16 cells were acquired from Millipore and used at P10-15 and expanded in DMEM/F-12 with 10% FBS (Gibco). Primary human ventricular cardiac fibroblasts were acquired from Promocell and expanded in FGM-3 (Promocell) and used at P3-5. For the experiment, cells were separately placed on tra...
ORGANISM(S): Homo Sapiens (human) 
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...3) with supplements (Promocell) then treated with 0.1 µM doxorubicin for 24 hours, then co-cultured through non-contact transwells with primary human ventricular fibroblasts (Promocell) with or without TGFb stimulation for 24 hours. The induction of senescence with doxorubicin and lack of spontaneous...
ORGANISM(S): Homo Sapiens (human) 
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