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TranscriptomicsCooperative blockade of CK2 and ATM kinases drives apoptosis in VHL-deficient renal carcinoma cells / RNA-seq (BRB-seq) of treated 786-O spheroids
Multicellular Tumor Spheroids (MCTS) were pre-formed for 3 days with 786-O cell line to better mimic the tumor microenviroenment. These spheroids were treated with either vehicle (DMSO), drugs alone (KU-60019 10 μM; CX-4945 5 μM) or in combination (KU + CX) for 48h before RNA extraction.
ORGANISM(S): Homo sapiens 
2021-02-04 | E-MTAB-9977 | biostudies-arrayexpress
TranscriptomicsBRBseq performed on 786-O cells treated with 2 inhibitors of protein kinase CK2, CX4945 and AB668
786-O cells (150, 000) were treated for 24h with DMSO, 5 µM of CX4945 or AB668. Total RNA was extracted from treated cells using the MirVana PARIS kit (Thermofisher). The 3′ Bulk RNA Barcoding and sequencing (BRB-seq) experiments were performed at the Research Institute for Environmental and Occupat...
ORGANISM(S): Homo sapiens 
2023-05-27 | E-MTAB-12776 | biostudies-arrayexpress
TranscriptomicsComparison of gene expression between 3 Renal Cell Carcinoma spheroids
RNA extracts from spheroids (786-O, RCC7 and RCC10) were obtained using the RNeasy Micro Kit (Qiagen) following manufacturer’s instructions. Five spheroids were used for each extract. High sensitivity Bulk RNA Barcoding (BRB)-sequencing and raw data preprocessing were performed by Alithea Genomics ...
ORGANISM(S): Homo sapiens 
2026-02-18 | E-MTAB-16624 | biostudies-arrayexpress
TranscriptomicsBRBseq performed on tumoroids and corresponding renal tumor tissues
To evaluate genetic similarities between tumoroids and the corresponding tumor tissues, we performed RNA sequencing of cells for each experimental condition. Since we injected human cancer cells into mice, cancer cells in the tumors were human while microenvironmental cells (i.e. Fibroblasts, Immune...
ORGANISM(S): Homo sapiens 
2023-06-30 | E-MTAB-12928 | biostudies-arrayexpress
TranscriptomicsDownregulation of CK2-Beta is sufficient to drive epithelial-to-mesenchymal transition through Snail 1 induction
To understand how CK2M-NM-2 silencing promotes a mesenchymal phenotype in human epithelial cells, like TGFM-NM-2 does, we have employed whole genome microarray expression profiling as a discovery platform to compare genes regulated in CK2M-NM-2-depleted cells and in TGFM-NM-2-treated cells. To do s...
ORGANISM(S): Homo sapiens 
2012-05-06 | E-GEOD-28569 | biostudies-arrayexpress
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