Sort   by:  
 Page size 
ProteomicsGlycoproteomics of Flagellin C9LY14 from Selenomonas sputigena
The Gram-negative, flagellated, anaerobic, crescent-shaped bacterium Selenomonas sputigena is a potential human periodontal pathogen. Information on its virulence factors and underlying pathogenicity mechanisms is scarce. Here we show for the first time that S. sputigena produces a diversely and hea...
ORGANISM(S): Escherichia coli Selenomonas sputigena ATCC 35185 
2018-01-24 | PXD005859 | Pride
Lc-msms Glycoproteomics Flagellin glycosylation Glycomics Periodontitis Selenomonas sputigena 
Genomicsflagellin
Flagellin diversity
PRJEB47543 | ENA
ProteomicsIdentification of flagellin peptides in N. benthamiana apoplastic fluid
Purified flagellin from Pseudomonas Pta6605 WT was supplemented with N. benthamiana apoplastic fluids from plants transiently expressing only p19 or p19 and bGAL1 (a galactosidase). By performing subsequent LC/MSMS we wanted to identify and quantify the levels of flagellin peptides released by endog...
ORGANISM(S): Nicotiana benthamiana Pseudomonas amygdali pv. tabaci 
2019-04-15 | PXD011823 | Pride
Pseudomonas Pta6605 Beta-galactosidase Flagellin 
ProteomicsC. difficile strain 630 flagellin C modifications
In proteomics, the study of post-translational modifications often relies on some type of enrichment strategy. Here, we show that an approach that is commonly used in phosphoproteomics (Immobilised Metal Affinity Chromatography (Fe3+-IMAC)) also enriches for peptides with a unique post-translational...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2022-04-04 | PXD029552 | Pride
C. difficile Phosphoproteomics Flagellin 
ProteomicsEvolution of paralogous multicomponent systems for site-specific O-sialylation of flagellin in Gram-negative and Gram-positive bacteria
Many bacteria decorate flagellin with sialic acid-like sugars such as pseudaminic acid (Pse) by O-glycosylation on serine or threonine residues. Evidence for sufficiency of sialylation by a conserved flagellin glycosyltransferase (fGT) system is lacking, presumably because of (a) missing component(s...
ORGANISM(S): Shewanella oneidensis (strain MR-1) Caulobacter crescentus (strain NA1000 / CB15N) 
2024-06-18 | PXD051215 | Pride
Pseudaminic acid Bacteria Glfm O-glycosylation Flagellin Maf 
ProteomicsRe-annotated role of beta-Galactosidase in Flagellin deglycosylation
Most Eukaryotes recognise flagellin as a signature of bacterial invasion. In contrast to animals, plants do not recognise flagellin proteins, but conserved peptides released from flagellin (Felix et al., 1999). However, these peptides (e.g. flg22) are folded and buried deeply inside the flagellin po...
ORGANISM(S): Nicotiana benthamiana Pseudomonas syringae pv. tomato 
2019-04-15 | PXD010461 | Pride
Viosamine Beta-galactosidase O-glycosylation Pseudomonas syringae Flagellin 
ProteomicsReleased peptide from flagellin cleavage by SBT5.2a
The aim of this experiment is to identify the released peptides from flagellin obtained afte cleavage and processing by SBT5.2
ORGANISM(S): Nicotiana benthamiana Pseudomonas amygdali pv. tabaci str. ATCC 11528 
2024-11-02 | PXD056369 | Pride
Plant immunity Pseudomonas syringae Subtilases Flg22 Flagellin Nicotiana benthamiana 
ProteomicsFlagellin (FliC) phosphorylation affects Type 2 protease secretion and biofilm dispersal in Pseudomonas aeruginosa PAO1
Protein phosphorylation has a major role in controlling the life-cycle and infection stages of bacteria. Proteome-wide occurrence of S/T/Y phosphorylation has been reported for many prokaryotic systems. Previously, we reported the phosphoproteome of Pseudomonas aeruginosa and Pseudomonas putida. In ...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2022-03-02 | PXD003645 | Pride
Phosphorylation Flagellin Lc-msms 
GenomicsRNA-sequencing from flagellin-stimulated intestinal organoids
RNA-sequencing from flagellin-stimulated intestinal organoids
PRJNA483138 | ENA
ProteomicsHarnessing the acceptor substrate promiscuity of Clostridium botulinum Maf glycosyltransferase to glyco-engineer mini-flagellin protein chimeras
In this study, we identified the O-glycosylation sites in deletion constructs of recombinant Clostridium botulinum flagellin (CbFla) expressed from the E. coli strain, EV136 (K1:K12 strains engineered by Prof. Eric Vimr’s group to accumulate CMP-sialic acid in the cytosol). The recombinant flagellin...
ORGANISM(S): Clostridium botulinum str. LANGELAN F 
2024-08-22 | PXD044191 | Pride
Ltq orbitrap velos pro ion-trap mass spectrometer Nonulosonic acid O-glycosylation Neu5ac Sialic acid Kdo Clostridium botulinum Gram positive Flagellin Geobacillus kaustophilus Maf 
Sort   by:  
 Page size