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Ribosomes that stall before completing peptide synthesis must be recycled and returned to the cytoplasmic pool. The protein Dom34 and cofactors Hbs1 and Rli1 can dissociate stalled ribosomes in vitro, but the identity of targets in the cell is unknown. Here we use ribosome profiling methodology to r...
ORGANISM(S): Saccharomyces cerevisiae 
During translation elongation, the ribosome ratchets along its mRNA template, incorporating each new amino acid and translocating from one codon to the next. The elongation cycle requires dramatic structural rearrangements of the ribosome. We show here that deep sequencing of ribosome-protected mRNA...
ORGANISM(S): Saccharomyces cerevisiae 
Nuclease footprints in sperm project past and future chromatin regulatory events
Post-transcriptional gene regulation plays a significant role in the response to oxygen deprivation. Here, we utilized advances in next-generation sequencing technology to examine changes in transcriptional control, mRNA loading on to polysome, and regulation of ribosome activity during mRNA transla...
ORGANISM(S): Arabidopsis thaliana 
Tracking of dCas9-methyltransferase footprints
Analysis of conditions leading to 3'UTR ribosome protected footprints
Chromatin footprints after double-hit stress
Nuclear remodeling to an extreme condensed state is a hallmark of spermatogenesis. This is achieved by varied degrees of replacement of histones with protamines. Regions retaining nucleosomes may be of functional significance. To determine potential roles for somatic-like chromatin in the paternal g...
ORGANISM(S): Homo sapiens Mus musculus 
2016-05-04 | GSE78075 | GEO
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