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ProteomicsIn vitro synthesis of 32 translation-factor proteins from a single template reveals impaired ribosomal processivity
The Protein synthesis Using Recombinant Elements (PURE) system enables transcription and translation of a DNA template from purified components. Therefore, the PURE system-catalyzed generation of RNAs and proteins constituting the PURE system itself represents a major challenge toward a self-replica...
ORGANISM(S): Escherichia Coli 
2021-09-06 | PXD020877 | panorama
ProteomicsTargeted proteomics measurements for Genetically controlled membrane synthesis in liposomes
Lipid membranes, nucleic acids, proteins, and metabolism are essential for modern cellular life. Synthetic systems emulating the fundamental properties of living cells must therefore be built upon these functional elements. In this work, phospholipid-producing enzymes encoded in a synthetic minigeno...
ORGANISM(S): Escherichia Coli 
2021-03-12 | PXD020399 | panorama
ProteomicsMin protein in vitro protein expression kinetics
The Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Cell-free assays relying on purified proteins have shown that MinE and MinD self-organize into surface waves on a planar membrane and into various oscillatory patterns in...
ORGANISM(S): Escherichia Coli 
2019-11-11 | PXD015686 | panorama
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