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ProteomicsNontargeted plasma proteomics analysis uncovers candidate biomarkers for renal disease and pulmonary hypertension in patients with sickle cell disease
Fifteen microliters of plasma from sickle cell disease patients, and pooled samples, were diluted with 5% deoxycholate and 10 mM DTT, followed by heating at 80 degC for 30 min, alkylation with 25 mM iodoacteamide and digestion with modified trypsin for 4 h at 37 degC. After acidification, samples we...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-01-19 | MSV000093893 | MassIVE
ProteomicsInterleukin 13 disrupts type 2 pneumocyte stem cell activity
Chronic lung disease is increasing in prevalence and there is urgent need to advance our understanding of human lung biology in order to improve diagnosis and current treatments. The Th2 inflammatory cytokine Interleukin 13 (IL13) has been associated with both obstructive and fibrotic lung diseases...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2020-06-05 | PXD013006 | Pride
Idiopathic pulmonary fibrosis Secretome Alveolar epithelial cell 
ProteomicsLayers of regulation on cell-cycle gene expression in the budding yeast Saccharomyces cerevisiae
In the budding yeast Saccharomyces cerevisiae, transcription factors (TFs) regulate the periodic expression of many genes during the cell cycle, including gene products required for progression through cell-cycle events. Experimental evidence coupled with quantitative models suggest that a network o...
ORGANISM(S): Saccharomyces Cerevisiae 
PXD010937 | panorama
ProteomicsLocalized K63 ubiquitin signaling is regulated by VCP/p97 during oxidative stress
Under stress conditions, cells reprogram their molecular machineries to mitigate damage and promote survival. Ubiquitin signaling is globally increased during oxidative stress, controlling protein fate and supporting stress defenses at several subcellular compartments. However, the rules driving sub...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-06-04 | MSV000094932 | MassIVE
ProteomicsPharmacokinetic analysis of a novel human EGFRvIII:CD3 bispecific antibody in plasma and whole blood using a high-resolution targeted mass spectroscopy approach
Bi-specific single change antibody fragments (bi-scFv) represent an emerging class of biotherapeutics. We recently developed a fully human bi-scFv (EGFRvIII:CD3 bi-scFv) with the goal of redirecting CD3-expressiong T cells to recognize and destroy malignant, EGFR-expressing glioma. In mice, we showe...
ORGANISM(S): Homo Sapiens Mus Musculus 
PXD012472 | panorama
ProteomicsPhosphoproteome of shUBE2N in melanoma cells
Quantitative label-free LC-MS/MS was performed on TiO2-enriched phosphopeptides from A375 cells expressing shUBE2N versus shcontrol (n=3 per group).
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-03-25 | MSV000082208 | MassIVE
MetabolomicsA female gametocyte-specific ABC transporter plays a role in lipid metabolism in the malaria parasite
ATP-binding cassette (ABC) transporters serve a variety of physiological functions as well as play key roles in drug resistance. The genome of the human malaria parasite, Plasmodium falciparum, encodes multiple members of this family, one of which, gABCG2, is transcribed predominantly in the gametoc...
2016-09-14 | MTBLS98 | MetaboLights
ProteomicsUrinary Proteome of Chronic Kidney Disease of Unknown Etiology (CKDu)
2 mL of preserved urine samples were precipitated overnight with 5 mL of ice-cold acetone, pellets were washed 70% acetone resuspended in 5% SDS followed by trypsin digestion of up to 100 ugs of each sample using an S-trap. One-third of tryptic peptides were microflow LC-MS/MS using an ACQUITY UPLC ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-10-11 | MSV000090513 | MassIVE
ProteomicsBeta-cell Galphas signaling is critical for physiological and pharmacological enhancement of insulin secretion
Islet lysates were adjusted to 5 percent SDS in TEAB, pH 8.5, reduced and alkylated and digested with modified trypsin (Worthington) using an S-trap mini device (Protifi). Peptides were labeled with TMT16 reagents (lot ZH350094). Phosphopeptide enrichment used GL Sciences p10 TiO tips. LC-MS/MS used...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2025-05-13 | MSV000097882 | MassIVE
ProteomicsVestibular schwannoma is comprised of neural crest and immune subgroups
Vestibular schwannomas (VS) are tumors arising from cranial nerve Schwann cells that cause significant morbidity and are treated with surgery and irradiation. We describe a DNA methylation-based classification of VS into neural crest and immune enriched subgroups and validate our findings using bulk...
ORGANISM(S): Homo Sapiens 
PXD014883 | panorama
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