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TranscriptomicsSingle cell RNA sequencing of CellTagging lung organoids
Here, we used a lentiviral-based barcoding method (CellTagging) to lineage trace progenitor cells in a human lung organoid model. 21-day cultures were transduced for 7 days, fluorescence activated cell sorted for GFP+ expression, and regrown as sparsely plated cells for additional 30 days before seq...
ORGANISM(S): Homo sapiens 
2023-04-21 | E-MTAB-12753 | biostudies-arrayexpress
TranscriptomicsSingle-nucleus RNA sequencing of healthy human adult lung from trachea, bronchi and bronchioles
We performed micro-dissections of adult human lung obtained through Gift of Life and performed single nuclear RNA-sequencing (n = 2 biological replicates, age 20, 26 yo) on trachea, bronchi, and bronchioles.
ORGANISM(S): Homo sapiens 
2025-08-01 | E-MTAB-14953 | biostudies-arrayexpress
TranscriptomicsSingle nucleus RNA sequencing of an in vitro primary adult human colonic epithelium at early, mid, and late time points during long-term culture
We developed a new in vitro model of primary adult human colonic epithelium on a transwell that maintains functional barrier for up to 30 days in culture. The epithelium on the 2-dimensional transwell was derived from normal primary adult human colonoid line Colon 88 at passage 9 (3-dimensional colo...
ORGANISM(S): Homo sapiens 
2025-08-01 | E-MTAB-15263 | biostudies-arrayexpress
TranscriptomicsSingle cell RNA sequencing of iPSC-derived lung organoid models
Here, we used single cell RNA sequencing of iPSC17 WT 7B2-derived day 10 lung spheroids, unsorted 3-, 6-, and 10-week lung progenitor organoids (LPOs) and induced bud tip progenitor organoids (iBTOs) sorted from 4- and 10-week LPOs, sequenced 4 weeks post-sort. All cultures were grown in serum-free ...
ORGANISM(S): Homo sapiens 
2022-07-24 | E-MTAB-11953 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal lung explants with R-SPONDIN Inhibition and human fetal lung primary tissue
Here, we used single cell RNA-sequencing (scRNA-seq) to profile 13-week post-conception distal human fetal lung explants cultured in an air liquid interface system and treated with an LGR5 ectodomain adenovirus that inhibits R-Spondin function or control adenovirus. A third, non-infected, condition ...
ORGANISM(S): Homo sapiens 
2022-01-01 | E-MTAB-10662 | biostudies-arrayexpress
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