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Formalin fixed paraffin-embedded pancreas tissues were deparaffinized by two changes of xylene and washed with descending concentrations of ethanol. Proteins in the deparaffinized tissues were extracted in 20%(w/v) of 200 mM Tris-HCl (pH 8.8) containing 2% SDS and 0.2 M DTT followed by incubation at...
ORGANISM(S): Homo Sapiens (human) 
CARASIL (cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy) is a hereditary cerebral small-vessel disease caused by loss of function mutations of HTRA1, which is a serine protease with a variety of targets, including extracellular matrix proteins. We isolate...
ORGANISM(S): Mus Musculus (mouse) 
CARASIL (cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy) is a hereditary cerebral small-vessel disease caused by loss of function mutations of HTRA1, which is a serine protease with a variety of targets, including extracellular matrix proteins. Htra1-KO m...
ORGANISM(S): Mus Musculus (mouse) 
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