HCT116 cells transiently transfected with FLAG-tagged RPS7, RPS17, or RPS19 expression vectors or empty vectors were fixed with 0.1% formaldehyde methanol-free (Pierce) and then lysed on ice in 1 ml of HEPES-RIPA2 buffer (20 mM HEPES-NaOH pH7.5, 1 mM EGTA, 1 mM MgCl2, 150 mM NaCl, 0.25% Na-deoxychol...
ORGANISM(S): Homo Sapiens (human)
