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ProteomicsLFQ of immunoprecipitates with GFP-Trap from GFP-, OfMasc-GFP-, or Del1-OfMasc-GFP-transfected cells
OfMasc-GFP derivatives or GFP were transiently expressed in OfTC cells. At 2 days post transfection, OfTC cells were treated with 100 nM Bortezomib for 1 day. The OfT1C cells were then fixed with 0.1% formaldehyde, and the fixation was quenched with 300 mM glycine. The cells were lysed on ice in 1 m...
ORGANISM(S): Cellular Organisms 
2022-10-04 | PXD034837 | JPOST Repository
ProteomicsLFQ of GFP-Trap IP-MS of OfMasc-interacting proteins
OfMasc-GFP derivatives or GFP were transiently expressed in OfT1C/tet cells (3.2e6 cells) seeded in 10 cm-diameter culture dishes. At two days post transfection, the cells were fixed, collected, and lysed on ice for 10 min in 1 mL of RIPA buffer (20 mM HEPES-KOH, pH 7.5, 1 mM EGTA, 1 mM MgCl2, 150 m...
ORGANISM(S): Cellular Organisms 
2025-10-25 | PXD059481 | JPOST Repository
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