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TranscriptomicsRescue of murine Gata1s mutant M7 leukemic cells by full-length Gata1
In this project, we studied a mouse model of human Down Syndrome (DS) megakaryocytic leukemia involving mutations in the GATA1 transcription factor (called GATA1s mutation). The model was generated through retroviral insertional mutagenesis in Gata1s mutant fetal liver progenitors. In this study, we...
ORGANISM(S): Mus musculus 
2010-08-01 | E-GEOD-16676 | biostudies-arrayexpress
TranscriptomicsInducible Gata1 Suppression Expands Erythro-Megakaryocytic Progenitors from Embryonic Stem Cells
Embryonic stem (ES) and induced pluripotent stem (iPS) cells represent a potential source of megakaryocytes and platelets for transfusion therapies. However, most current ES/iPS cell differentiation protocols are limited by low yields of hematopoietic progeny. Mutations in the mouse and human genes ...
ORGANISM(S): Mus musculus 
2015-12-09 | E-GEOD-66479 | biostudies-arrayexpress
GenomicsGenome-wide maps of GATA1 occupany in induced G1E ER4 cell line.
we mapped the locations of DNA segments occupied by GATA1 using chromatin immunoprecipitation (ChIP). We have produced genome-wide GATA1 ChIP datasets after restoration and activation in G1E-ER4 cells. we employed the sequence census methodology of ChIP-seq , using Illumina GA2 technology to produ...
ORGANISM(S): Mus musculus 
2009-09-19 | E-GEOD-18164 | biostudies-arrayexpress
TranscriptomicsDevelopmental stage-specific interplay between GATA1 and IGF signaling in fetal hematopoiesis and leukemogenesis
This SuperSeries is composed of the following subset Series: GSE16676: Rescue of murine Gata1s mutant M7 leukemic cells by full-length Gata1 GSE16677: Gene expression profiling of Down Syndrome (DS)-AMKL and non-DS AMKL samples GSE16679: Plag1 overexpression cooperates with Evi1 overexpression and G...
ORGANISM(S): Mus musculus 
2010-08-01 | E-GEOD-16655 | biostudies-arrayexpress
TranscriptomicsConsequences of GATA1 expression in Gata1- G1ME Murine Megakaryocyte/Erythrocyte Progenitor Cell Line
G1ME cells are GATA1-deficient murine bipotential megakaryocyte/erythrocyte progenitor cells derived from Gata1-negative murine ES cells. In order to assess the impact of GATA1 on gene regulation and cell differentiation, an expression construct was used to transiently produce high levels of GATA1....
ORGANISM(S): Mus musculus 
2009-06-10 | E-GEOD-14980 | biostudies-arrayexpress
TranscriptomicsTranscriptome profiling of mouse Gata1- megakaryocyte-erythroid progenitors (G1MEs) expressing one of the two isoforms of GATA1: full-length (GATA1fl) or an amino-truncated form of GATA1 (GATA1s).
We transduced mouse Gata1- megakaryocyte-erythroid progenitors with MIGRI-GFP vector expressing GATA1fl or GATA1s cDNAs. GFP-positive cells expressing one of the two isoforms of GATA1 were isolated by FACS 42 hours following transduction and used for microarray transcriptome analysis. At this time p...
ORGANISM(S): Mus musculus 
2015-01-26 | E-GEOD-62879 | biostudies-arrayexpress
GenomicsGenome-wide transcription factor binding and chromatin methylation marks in the G1ME megakaryocytic progenitor model cell line
There are many examples of transcription factor families whose members control gene expression profiles of diverse cell types. However, the mechanism by which closely related factors occupy distinct regulatory elements and impart lineage specificity is largely undefined. Here we demonstrate on a gen...
ORGANISM(S): Mus musculus 
2012-03-05 | E-GEOD-31331 | biostudies-arrayexpress
GenomicsLdb1-nucleated transcription complexes function as primary mediators of erythroid gene activation
We used ChIP-Seq to map Ldb1, Scl and Gata1 binding sites in mouse total bone marrow cells. Together with functional studies comparing gene expression in Murine Erythroleukemia (MEL) cells expressing Ldb1 shRNA or control shRNA and bioinformatics analysis, we systematically determined the transcrip...
ORGANISM(S): Mus musculus 
2013-04-29 | E-GEOD-42843 | biostudies-arrayexpress
GenomicsGenome-wide analysis of GATA1, GATA2, RUNX1, FLI1 and SCL binding in primary human megakaryocytes
We have mapped the binding sites for the five key regulators GATA1, GATA2, RUNX1, FLI1 and TAL1/SCL in primary human megakaryocytes. Statistical analysis identified subsets of enriched as well as depleted combinatorial binding patterns. In particular simultaneous binding by all 5 factors was highly ...
ORGANISM(S): Homo sapiens 
2011-06-01 | E-GEOD-24674 | biostudies-arrayexpress
TranscriptomicsTAF10 interacts with the GATA1 transcription factor and controls mouse erythropoiesis
We have ablated TAF10 in the erythroid compartment only by crossing the TAF10lox mice with the EpoR-Cre mice and we have studied the development of the erythroid cells in vivo. TAF10 ablation led to embryonic death at E13.5 while at E12.5 there was a clear developmental defect which was reflected in...
ORGANISM(S): Mus musculus 
2015-04-21 | E-GEOD-68083 | biostudies-arrayexpress
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