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ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
ProteomicsA new method for differential proteomic analysis utilizing native MS-electropherograms: a comparison between plasma and serum proteins by combining native PAGE, whole-gel slicing and quantitative LC-MS/MS
MS identification has long been used for PAGE-separated protein bands, but global and systematic quantitation utilizing MS after PAGE has remained rare and not been reported for native PAGE. Here we reported on a new method combining native PAGE, whole-gel slicing and quantitative LC-MS/MS aiming c...
ORGANISM(S): Homo sapiens (Human) 
2017-09-08 | PXD006773 | Pride
Gel slicing Quantitative lc-ms/ms Native ms-electropherogram Native 1d-page Human plasma and serum Differential proteomic analysis 
ProteomicsCryo-slicing BN-MS of rat brain mitochondrial proteins
Blue-native gel electrophoresis (BN) is a powerful method for protein separation. Combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) it enables large-scale identification of protein complexes and their subunits. Current BN-MS approaches, however, are limited in size resolution, ...
ORGANISM(S): Rattus norvegicus (Rat) 
2015-12-01 | PXD002681 | Pride
Cryo-slicing bn-ms lc-ms/ms complexome proteine profiles 
Proteomics1DE-MS profiling of rat cerebral cortex proteins of focal cerebral ischemia-reperfusion injury by combining SDS-PAGE, gel slicing, quantitative LC-MS/MS and profile reconstruction
A rat model of focal cerebral ischemia-reperfusion (I/R) injury was established by 2 hour-middle cerebral artery occlusion (MCAO) followed with 1, 7 and 14 days of reperfusion. Validation of the model was done by the measurement on cerebral blood flow, infarct volume, histological damage and neurol...
ORGANISM(S): Rattus Norvegicus 
2022-05-11 | PXD033793 |
UnknownCryo-slicing Blue Native-Mass Spectrometry (csBN-MS), a Novel Technology for High Resolution Complexome Profiling.
Not available
S-EPMC4739680 | biostudies-literature
TranscriptomicsPIWI slicing and EXD1 drive biogenesis of nuclear piRNAs from cytosolic targets of the mouse piRNA pathway
PIWI-interacting RNAs (piRNAs) guide PIWI proteins to suppress transposable elements in animal gonads. Here we demonstrate that in the mouse embryonic male germline, endonucleolytic cleavage (slicing) of a transcript by cytosolic MILI acts as a trigger to initiate its further 5??3? processing into n...
ORGANISM(S): Mus musculus 
2015-12-04 | E-GEOD-74423 | biostudies-arrayexpress
ProteomicsProteomic analysis of focal cerebral ischemia/reperfusion injury in rats
Stroke is a leading cause of mortality and long-term disability and ischemic stroke accounts for 87% of all strokes. Though timely recanalization of the occluded vessel is essential in the treatment of ischemic stroke, it is well known to cause ischemia-reperfusion (I/R) injury which result in neuro...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-07-03 | PXD012151 | Pride
Middle cerebral artery occlusion (mcao) Proteomics Rat Time course Brain stroke Ischemia-reperfusion injury 
TranscriptomicsRNA clamping by Vasa assembles a piRNA Amplifier complex on transposon transcripts
Germline-specific Piwi-interacting RNAs (piRNAs) protect the genome against selfish genetic elements and are essential for fertility in animals. piRNAs targeting active transposons are amplified by a feed-forward loop known as the Ping-pong cycle, which links endonucleolytic slicing of target RNA...
ORGANISM(S): Bombyx mori 
2014-06-04 | E-GEOD-57420 | biostudies-arrayexpress
TranscriptomicsFunctional analysis of Arabidopsis ARGONAUTE1 using a slicer-defective mutant: Small RNA immunoprecipitation
The Arabidopsis ARGONAUTE (AGO) protein AGO1 associates with microRNA (miRNA) and specific classes of short-interfering RNA (siRNA). AGO1-small RNA complexes recognize target RNA transcripts through base-pairing interactions and inhibit translation of target RNAs through endonucleolytic cleavage (sl...
ORGANISM(S): Arabidopsis thaliana 
2012-09-28 | E-GEOD-40258 | biostudies-arrayexpress
ProteomicsIdentification of SENP6 targets in HeLa cells by SENP6 SiRNA and MW shift analysis
The SUMO protease SENP6 disassembles SUMO chains and thus SENP6 depletion should lead to the extension of SUMO polymers on substrates. We have used a proteomic method to identify putative SENP6 substrates based on increased apparent molecular weight after SENP6 depletion. This method uses SDS-PAGE f...
ORGANISM(S): Homo sapiens (Human) 
2024-03-01 | PXD036743 | Pride
Sumo senp6 molecular weight 
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