Mass spectrometry-based proteomics sample preparation
Harvested HUVECs, approximately 5 million cells each, were pelleted and frozen at -80C. The sample pellet was lysed according to the Filter Aided Sample Preparation (FASP) protocol.59 Lysis buffer used in the FASP was changed to 6% SDS, 150 mM DT...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
