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TranscriptomicsRiboSeq of African green monkey (Vero) cells infected at a multiplicity of infection (MOI) of 3 with the Asian/American Zika virus (ZIKV) strain, PE243
Vero cells were infected with the Asian/American Zika virus (ZIKV) strain, PE243, at MOI:3 to assess the viral transcriptome. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide pre-treatment. RNase I treatment was carried out, following which ribosome...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12971 | biostudies-arrayexpress
TranscriptomicsRiboSeq of African green monkey (Vero) cells infected at a multiplicity of infection (MOI) of 3 with different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics
Vero cells were infected with the different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics at MOI:3 to assess their viral transcriptome. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide p...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12969 | biostudies-arrayexpress
TranscriptomicsRiboSeq of African green monkey (Vero) cells and human malignant glioblastoma multiforme (U251) cells infected at a multiplicity of infection (MOI) of 3 with the Asian/American Zika virus (ZIKV), PE243; and the African ZIKV strain, Dak84
Vero and U251 cells were infected with the Asian/American ZIKV (PE243) and the African ZIKV (Dak84) at MOI:3 to assess the viral transcriptome in two cell lines. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide pre-treatment. RNase I treatment was ...
ORGANISM(S): Homo sapiens 
2024-09-04 | E-MTAB-12970 | biostudies-arrayexpress
TranscriptomicsColorectal tumor DNA vs. normal control DNA
Genomic DNA samples were extracted from microdissected colorectal tumour tissue and then compared against normal DNA extracted from the peripheral blood from the same patients. 53 Normal/Tumor matched samples.
ORGANISM(S): Homo sapiens 
2012-10-25 | E-GEOD-41813 | biostudies-arrayexpress
UnknownNivolumab for Metastatic Renal Cell Carcinoma: Results of a Randomized Phase II Trial.
Not available
S-EPMC4806782 | biostudies-literature
MetabolomicsMitochondrial metabolism sustains DNMT3A-R882-mutant clonal haematopoiesis
Somatic DNMT3A R882 codon mutations drive the most common form of clonal haematopoiesis (CH) and are associated with increased acute myeloid leukaemia (AML) risk1,2. Preventing expansion of DNMT3A-R882-mutant haematopoietic stem/progenitor cells (HSPCs) may therefore avert progression to AML. To ide...
2025-02-06 | MTBLS12201 | MetaboLights
TranscriptomicsRNASeq of African green monkey (Vero) cells infected at a multiplicity of infection (MOI) of 3 with different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics
Vero cells were infected with the different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics at MOI:3 to assess their viral transcriptome. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide p...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12968 | biostudies-arrayexpress
TranscriptomicsWhole genome \"TraDIS-Xpress\" assay to determine gene roles during growth of E. coli in different concentrations of the antibiotics trimethoprim and sulfamethoxazole
To assay every gene in the E. coli genome to identify those that contribute to increased or decreased susceptibility to the antibiotics trimethoprim and sulfamethoxazole. This will help to define more accurately those bacterial cell mechanisms that contribute to these phenomena and provide informat...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2020-10-19 | E-MTAB-9305 | biostudies-arrayexpress
TranscriptomicsRNASeq of African green monkey (Vero) cells and human malignant glioblastoma multiforme (U251) cells infected at a multiplicity of infection (MOI) of 3 with the Asian/American Zika virus (ZIKV), PE243; and the African ZIKV strain, Dak84
Vero and U251 cells were infected with the Asian/American ZIKV (PE243) and the African ZIKV (Dak84) at MOI:3 to assess the viral transcriptome in two cell lines. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide pre-treatment. Ribosomal RNA was remov...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12967 | biostudies-arrayexpress
TranscriptomicsJHI Pa MC mutants
Plant cell wall degrading enzymes (PCWDEs) are key virulence determinants in the pathogenesis of the potato pathogen, Pectobacterium atrosepticum. In this study, we report the impact on virulence of a transposon insertion mutation in the metJ gene that codes for the repressor of the methionine biosy...
ORGANISM(S): Pectobacterium atrosepticum 
2012-11-02 | E-MTAB-1167 | biostudies-arrayexpress
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