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TranscriptomicsKhd1p associated mRNAs
RNA-coimmunopurifications with TAP-tagged Khd1 protein from Saccharomyces cereviseae. Untagged strain (BY4741) served as a control (Gerber et al., 2004). Cells were grown to midlog phase in rich media and harvested by centrifugation. TAP-tagged Khd1 was affinity purified from cell-free extracts with...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-20 | E-GEOD-10279 | biostudies-arrayexpress
TranscriptomicsPuf proteins, RNA co-immunopurification
RNA-coimmunopurifications with TAP-tagged Puf proteins from Saccharomyces cereviseae. Untagged strain (BY4741) served as a control. Cells were grown to midlog phase and harvested by centrifugation. TAP-tagged Puf proteins were affinity purified from cell-free extracts with IgG sepharose and eluted ...
ORGANISM(S): Saccharomyces cerevisiae 
2006-01-03 | E-GEOD-3968 | biostudies-arrayexpress
TranscriptomicsShg1p associated RNAs
RNA co-immunopurification with TAP-tagged Shg1p (a component of the Set1 histone methyltransferase complex, SET1C) from Saccharomyces cerevisiae. An untagged (BY4741) served as a control (Gerber et al. 2004). Cells were grown to mid-log phase in rich media and harvested by centrifugation. TAP-tagged...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-24 | E-GEOD-15247 | biostudies-arrayexpress
TranscriptomicsRBP40_arrays
In this study, we systematically identified the RNAs associated with a selective sample of 40 of ~600 yeast RNA-binding proteins (RBPs). To identify RNAs associated with each putative RBP, C-terminal tandem affinity purification (TAP)-tagged proteins, expressed under control of their native promoter...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-25 | E-GEOD-13135 | biostudies-arrayexpress
TranscriptomicsSlf1p and Sro9p affinity isolations
To identify RNAs specifically associated with Slf1p and Sro9p, cells expressing TAP-tagged Slf1, Sro9 or Fpr1 (negative control) were grown to mid-log phase in synthetic complete medium and harvested by centrifugation. RNA affinity isolations were essentially performed as described (Gerber et al. 20...
ORGANISM(S): Saccharomyces cerevisiae 
2012-02-08 | E-GEOD-27310 | biostudies-arrayexpress
TranscriptomicsRNA-affinity isolations 13 RBPs
To identify RNAs specifically associated with potential RBPs, yeast cells expressing TAP-tagged RBP or the wild-type strain BY4741 (mock control) were grown to mid-log phase in rich medium and harvested by centrifugation. RNA affinity isolations were essentially performed as described (Gerber et al...
ORGANISM(S): Saccharomyces cerevisiae 
2010-12-06 | E-GEOD-21864 | biostudies-arrayexpress
TranscriptomicsRNA affinity isolation with TAP-Pum and controls
Biochemical purification: Five grams of adult flies or 2.5 g of embryos were used in each affinity purification. Flies or embryos were suspended in 15 ml of buffer B (buffer A plus 1.5 mM dithiothreitol [DTT], 1 mM phenylmethylsulfonylfluoride [PMSF], 0.5 5g/ml leupeptin, 0.8 5g/ml pepstatin, 20 U/m...
ORGANISM(S): Drosophila melanogaster 
2006-02-28 | E-GEOD-3582 | biostudies-arrayexpress
TranscriptomicsmRNAs associated with human PUM1 protein
To identify mRNAs associated with human PUM1 protein, we used a modified Ribonucleoprotein-ImmunoPrecipitation Microarray (RIP-Chip) approach on HeLa S3 cancer cells that express PUM1. PUM ribonucleoprotein (RNP) complexes were captured from cell-free extracts with anti PUM1 specific antibody Bethyl...
ORGANISM(S): Homo sapiens 
2008-09-08 | E-GEOD-12351 | biostudies-arrayexpress
TranscriptomicsmRNAs associated with human PUM1 and PUM2 protein
This SuperSeries is composed of the following subset Series: GSE12351: mRNAs associated with human PUM1 protein GSE12352: mRNAs associated with human PUM2 protein Refer to individual Series
ORGANISM(S): Homo sapiens 
2008-09-08 | E-GEOD-12357 | biostudies-arrayexpress
TranscriptomicsWild-type versus trf4, trf5, and trf4-DADA mutant cells
Measurement of the relative changes of gene expression of S. cerevisiae cells lacking either trf4 (trf4delta) or trf5 (trf5delta), and trf4delta/TRF4-DADA mutants compared to wild-type (WT) cells using yeast oligo microarrays that contain features representing all annotated yeast ORFs, ncRNAs, intro...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-24 | E-GEOD-16103 | biostudies-arrayexpress
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