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MetabolomicsLipopolysaccharides from Ralstonia solanacearum induce a broad metabolomic response in Solanum lycopersicum

Ralstonia solanacearum, one of the most destructive crop pathogens worldwide, causes bacterial wilt disease in a wide range of host plants. The major component of the outer membrane of Gram-negative bacteria, lipopolysaccharides (LPS), has been shown to function as elicitors of plant defense lead...

2023-08-08 | MTBLS7324 | MetaboLights
TranscriptomicsRNA co-immunoprecipitations with yeast She proteins
RNA co-immunoprecipitations with C-terminal protein A-tagged She proteins. One liter of cells were cultured at 30 degrees in YPAD medium and collected during exponential growth by centrifugation. Cells were washed twice and broken mechanically with glass beads. Extracts were incubated with IgG-agar...
ORGANISM(S): Saccharomyces cerevisiae 
2005-12-20 | E-GEOD-3879 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation with yeast She proteins
RNA co-immunoprecipitations with C-terminal protein A-tagged She proteins. One liter of cells were cultured at 30 degrees in YPAD medium and collected during exponential growth by centrifugation. Cells were washed twice and broken mechanically with glass beads. Extracts were incubated with IgG-agar...
ORGANISM(S): Saccharomyces cerevisiae 
2005-10-14 | E-SMDB-2092 | biostudies-arrayexpress
TranscriptomicsRNA affinity isolation with TAP-Pum and controls
Biochemical purification: Five grams of adult flies or 2.5 g of embryos were used in each affinity purification. Flies or embryos were suspended in 15 ml of buffer B (buffer A plus 1.5 mM dithiothreitol [DTT], 1 mM phenylmethylsulfonylfluoride [PMSF], 0.5 5g/ml leupeptin, 0.8 5g/ml pepstatin, 20 U/m...
ORGANISM(S): Drosophila melanogaster 
2006-02-28 | E-GEOD-3582 | biostudies-arrayexpress
TranscriptomicsTrf4p in vivo crosslinking and ribonucleoprotein-immunopurification-chip analysis (X-RIP-Chip)
In vivo cross-linking and ribonucleoprotein-immunopurification experiments followed by microarray analysis of bound RNAs (X-RIP-chip). Cells expressing recombinant tandem-affinity purification (TAP)-tagged Trf4 protein were cross-linked with formaldehyde, and Trf4-containing ribonucleoprotein comple...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-24 | E-GEOD-16105 | biostudies-arrayexpress
TranscriptomicsmRNAs associated with human PUM2 protein
To identify mRNAs associated with human PUM2 protein, we used a modified Ribonucleoprotein-ImmunoPrecipitation Microarray (RIP-Chip) approach on HeLa S3 cancer cells that express PUM2. PUM ribonucleoprotein (RNP) complexes were captured from cell-free extracts with specific anti PUM2 antibody couple...
ORGANISM(S): Homo sapiens 
2008-09-08 | E-GEOD-12352 | biostudies-arrayexpress
UnknownLevels of Breast Milk MicroRNAs and Other Non-Coding RNAs Are Impacted by Milk Maturity and Maternal Diet.
Not available
S-EPMC8796169 | biostudies-literature
UnknownInfant Saliva Microbiome Activity Modulates Nutritional Impacts on Neurodevelopment.
Not available
S-EPMC10459261 | biostudies-literature
TranscriptomicsDownstream targets of Fgfrl1 signaling
Comparison of the gene expression profiles between normal and Fgfrl1 deficient kidneys 3 individual RNA preparations from 6-9 pooled normal kidneys each vs. 3 individual RNA preparations from 6-9 Fgfrl1-/- kidneys each
ORGANISM(S): Mus musculus 
2012-03-16 | E-GEOD-32013 | biostudies-arrayexpress
UnknownTherapeutic 6-thio-deoxyguanosine inhibits telomere elongation in cancer cells by inducing a non-productive stalled telomerase complex.
Not available
S-EPMC12764492 | biostudies-literature
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