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ProteomicsProteome of mass-produced Escherichia coli outer membrane vesicles
Large-scale production of bacterial extracellular vesicles is a hurdle for their development as novel cancer immunotherapeutic agents. Here, we developed manufacturing processes for mass production of Escherichia coli EVs known as outer membrane vesicles (OMVs). We present a comprehensive proteome o...
ORGANISM(S): Escherichia coli Bacteria 
2023-10-24 | PXD043119 | Pride
Escherichia coli Outer membrane vesicles Mass production Lc-ms/ms 
ProteomicsEscherichia coli msbB-deficient mutant extracellular vesicle proteome
Bacteria actively secrete extracellular vesicles (EVs), spherical nano-sized proteolipids into the extracellular milieu. Bacterial EVs have gained wide interests as non-living complex vaccines or delivery vehicles. However, no studies have used bacterial EVs in treating cancer so far. Our results sh...
ORGANISM(S): Escherichia coli 
2018-11-22 | PXD006912 | Pride
Exosomes Escherichia coli Extracellular vesicles Outer membrane vesicles Bacteria 
ProteomicsStaphylococcus aureus RN4220 wild-type extracellular vesicle proteome
Bacteria actively secrete extracellular vesicles (EVs), spherical nano-sized proteolipids into the extracellular milieu. Bacterial EVs have gained wide interests as non-living complex vaccines or delivery vehicles. However, no studies have used bacterial EVs in treating cancer so far. Our results sh...
ORGANISM(S): Staphylococcus aureus 
2018-11-22 | PXD006913 | Pride
Exosomes Membrane vesicles Extracellular vesicles Bacteria Staphylococcus aureus 
ProteomicsP. aeruginosa OMV proteome - Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa
Pseudomonas aeruginosa, an opportunistic human bacterial pat hogen, constitutively secretes outer membrane vesicles (OMVs ) into the extracellular milieu. In this study, we identifi ed vesicular proteins with high confidence by five separate LC-MS/MS analyses.
ORGANISM(S): Pseudomonas aeruginosa 
2011-08-11 | PRD000293 | Pride
ProteomicsNetwork organization of the human exosomes
Exosomes, nano-sized bilayered proteolipids, play multiple roles in intercellular communication. Although many exosomal proteins have been identified, their functional interrelationships and the mechanisms of exosome biogenesis remain unknown. By interrogating proteomic data using systems approaches...
ORGANISM(S): Homo sapiens (Human) 
2012-02-09 | PRD000414 | Pride
ProteomicsQuantitative proteomic analysis of trypsin-treated extracellular vesicles to evaluate non-vesicular contaminants and surface vesicular proteins.
Extracellular vesicles (EVs) are lipid bilayered vesicles released into extracellular milieu by most of cells. Currently, various purification methods of EVs have been established but most of studies use ultracentrifugation-based purification of EVs. However, these methods have the problem of co-pur...
ORGANISM(S): Homo sapiens (Human) 
2021-08-30 | PXD001355 | Pride
Non-vesicular contaminants Quantitative proteomics Extracellular vesicles Trypsin treatment 
ProteomicsMycobacterium tuberculosis extracellular vesicle proteome
Tuberculosis is a common infectious disease mostly caused by Mycobacterium tuberculosis. M. tuberculosis have been shown to release extracellular vesicles (EVs) containing immunologically active molecules. However, only limited number of proteins has been identified in M. tuberculosis EVs. We presen...
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2015-07-30 | PXD001160 | Pride
Exosomes Mycobacterium tuberculosis Extracellular vesicles Outer membrane vesicles Bacteria 
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