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A hallmark of the biofilm architecture is the presence of microcolonies. However, little is known about the underlying mechanisms governing microcolony formation. In the human pathogen Pseudomonas aeruginosa, microcolony formation is dependent on the two-component regulator MifR, with mifR mutant bi...
ORGANISM(S): Pseudomonas aeruginosa 
Biofilms are surface-adhered bacterial communities encased in an extracellular matrix composed of polysaccharides, proteins, and extracelluar (e)DNA, with eDNA being required for the formation and integrity of biofilms. Here we demonstrate that the spatial and temporal release of eDNA is regulated b...
ORGANISM(S): Pseudomonas aeruginosa 
As part of the ENCODE consortium the GENCODE project is producing a reference gene set through manual and automated gene prediction. Selected transcript models are verified experimentally by RT-PCR amplification followed by sequencing. This is batch II, based on annotation from April 2009. ArrayExpr...
ORGANISM(S): Homo sapiens 
2010-12-04 | GSE25711 | GEO
As part of the RNASeq Genome Annotation Assessment Project (RGASP) selected novel gene model predictions were analysed with RT-PCR-Seq. ArrayExpress Release Date: 2011-03-01 Publication Title: GENCODE: producing a reference annotation for ENCODE Publication Author List: Harrow J, Denoeud F, Frankish...
ORGANISM(S): Homo sapiens Caenorhabditis elegans 
2011-07-14 | GSE30612 | GEO
As part of the ENCODE consortium the GENCODE project is producing a reference gene set through manual and automated gene prediction. Selected transcript models are verified experimentally by RT-PCR amplification followed by sequencing. This is batch VII, which targets annotations from Gencode 8 (Mar...
ORGANISM(S): Homo sapiens 
2012-04-25 | GSE37592 | GEO
As part of the ENCODE consortium the GENCODE project is producing a reference gene set through manual and automated gene prediction. Selected transcript models are verified experimentally by RT-PCR amplification followed by sequencing. This is batch V, based on annotations from Gencode 6 (November20...
ORGANISM(S): Homo sapiens 
2012-01-04 | GSE34820 | GEO
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