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ProteomicsAn automated ‘cells-to-peptides’ sample preparation workflow for high-throughput, quantitative proteomic assays of microbes
Mass spectrometry–based quantitative proteomic analysis has proven valuable for clinical and biotechnology related research and development. Driving this value have been improvements in the sensitivity, resolution, and robustness of mass analyzers. However, manual sample preparation protocols are of...
ORGANISM(S): Escherichia Coli Rhodotorula Toruloides Pseudomonas Putida Kt2440 Saccharomyces Cerevisiae 
PXD014182 | panorama
ProteomicsComprehensive genome-scale and pathway engineering for production of the sustainable aviation fuel in Pseudomonas putida
Biological production of aviation fuels has a realistic impact on global warming. Important sustainable aviation fuel (SAF) targets are emerging and isoprenol is recently identified as a precursor for a promising SAF compound DMCO (1,4-dimethylcyclooctane). Isoprenol has been produced in several eng...
ORGANISM(S): Pseudomonas Putida Kt2440 
PXD039868 | panorama
ProteomicsProgrammable polyketide biosynthesis platform for production of aromatic compounds in yeast
To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new, versatile, bio-based production platforms are required. Here we present a novel yeast platform for bi...
ORGANISM(S): Saccharomyces Cerevisiae 
2020-02-11 | PXD013388 | panorama
ProteomicsMassively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism
The lysine metabolism of Pseudomonas putida can produce multiple important commodity chemicals and is implicated in rhizosphere colonization. However, despite intensive study, the biochemical and genetic links between lysine metabolism and central metabolism remains unresolved in P. putida. Here, we...
ORGANISM(S): Pseudomonas Putida Kt2440 
PXD011350 | panorama
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