Expression, crosslinking and immunoprecipitation of tagged HNRPA2B1 (variant A2) to identify bound RNA sequences RNA molecules that are immunoprecipitated are sequenced followed purification under denaturing conditions
Transcriptional profiling of MDA-MB-231 samples taken at 0, 1, 2 and 4hr timepoints upon exposure to alpha-amanitine, comparing the siRNA-mediated HNRPA2B1 knock-downs versus mock-transfected controls 4 biological replicates for each time point for the siRNA transfections and controls respectively
