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We identified two novel transcription factors ‘Triterpene Saponin Activation Regulator’ (TSAR) 1 and 2 that each activate a specific branch of saponin synthesis in M. truncatula. Therefore we generated three independent TSAR1 and three independent TSAR2 overexpression (OE) hairy root lines. As a con...
ORGANISM(S): Medicago truncatula 
In hairy roots of the model legume Medicago truncatula the saponin production can be elicited by methyl jasmonate treatment. To identify genes potentially involved in saponin biosynthesis or its regulation we carried out transcript profiling by RNA-Seq of M. truncatula hairy roots treated with 100 μ...
ORGANISM(S): Medicago truncatula 
RNA-Seq analysis on Medicago truncatula hairy roots, either control lines or lines silenced for the Makbishi1 (MKB1) gene, grown in either mock conditions or elicited with the plant stress hormone methyl jasmonate for 4 hours
ORGANISM(S): Medicago truncatula 
A major role of NINJA is to repress root jasmonate signalling and allow normal cell elongation. To further characterize the transcriptional changes occurring in the root in the absence of a NINJA-dependent repression mechanism, we conducted a microarray analysis of ninja-1 versus WT roots. Root tran...
ORGANISM(S): Arabidopsis thaliana 
RNA sequencing was performed to compare the gene expression in tomato hairy root lines transformed with the inducible rolB, rolBmEAR2 or GFP overexpression constructs, 24 h after mock and β-estradiol treatments. This set-up allowed us to map the general RolB targets as well as the specific targets o...
ORGANISM(S): Solanum lycopersicum 
The experiment aims to identify genes encoding enzymes involved in the biosynthesis of triterpenoid saponins in Saponaria officinalis. RNA-seq data generated were generated from leaves, stems, and roots from 3 months old- hydroponically grown S. officinalis plants that were treated with 50 µM methyl...
ORGANISM(S): Saponaria officinalis 
RNA sequencing was performed on tomato hairy root lines transformed with a GUS overexpression construct, 24 h after mock and jasmonate (JA) treatment. This set-up allowed us to map the gene targets of JA in tomato hairy roots after 24h.
ORGANISM(S): Solanum lycopersicum 
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