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Mass spectrometry analysis of NeuN-positive neuronal nuclei from cortices of young and aged mice
ORGANISM(S): Mus Musculus (mouse) 
To identify PTMs of MAVS, we overexpressed His-Flag-tagged hMAVS 1-200 in 293T cells and cell lysates were analyzed by LC-MS/MS.
ORGANISM(S): Homo Sapiens (human) 
To identify PTMs of MAVS, we overexpressed His-Flag-tagged hMAVS 1-200 in 293T cells and cell lysates were analyzed by LC-MS/MS.
ORGANISM(S): Homo Sapiens (human) 
To identify proteins that differentially interact with carboxylated and non-carboxylated MAVS, we overexpressed Flag-tagged WT or 4A mutant (D53A, E70A, E80A, and D83A) forms of hMAVS in 293T cells and analyzed the interactors using immunoprecipitation followed by mass spectrometry (MS). Cell lysate...
ORGANISM(S): Homo Sapiens (human) 
Pdzd8-3xHA knock-in mice and control littermates at postnatal 10 days were put to sleep using medetomidine hydrochloride, midazolam and butorphanol. Pups were then put on the ice for 5 minutes and exsanguinated by terminal intracardial perfusion with ice-cold 2% paraformaldehyde in PBS. The neocorte...
ORGANISM(S): Mus Musculus (mouse) 
PDZD8-TurboID knocked-in HeLa cells were plated into a 15 cm dish and cultured two overnight. The cells were treated with Biotin at the final concentration of 50 μM, and incubated for 6 hours. The cells were washed twice with ice-cold Hepes-saline and lysed in 6 M guanidine-HCl containing 100 mM HEP...
ORGANISM(S): Homo Sapiens (human) 
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