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ProteomicsThe hypothalamus is an early site of mitochondrial failure and neuro-immune circuit disruption in amyotrophic lateral sclerosis
Given the hypothalamus central role in energy homeostasis and its emerging vulnerability in ALS, this study aimed to determine whether hypothalamic mitochondrial dysfunction may contribute to and potentially drive disease-related metabolic disturbances, thereby representing a potential therapeutic t...
ORGANISM(S): Mus musculus (Mouse) 
2026-04-06 | PXD070931 | Pride
Proteomics Mitochondria Als 
ProteomicsSequential Fractionation Strategy Identifies Nine Missing Proteins in The Mitochondrial Proteome of Commonly Used Cell Lines
Identification of mitochondrial proteins with a bottom up approach after organelle enrichment and 1D PAGE separation for 5 different cell lines. The bands were cut and the proteins reduced, alkylated and digested with trypsin according to a conventional protocol. Acquisition were performed on the Or...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-05-28 | MSV000082409 | MassIVE
ProteomicsStandardization of mitochondrial proteomics v2 NP
Comparison of the extraction performances of three different mitochondria enrichment protocols (differential centrifugation, sucrose gradient, a commercial kit based on surfactants) on ten different cell lines. Samples were analysed on several instrumental platforms. Mitochondrial pellets were lysed...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-07-14 | MSV000081329 | MassIVE
ProteomicsStandardization of mitochondrial proteomics
Comparison of the extraction performances of three different mitochondria enrichment protocols (differential centrifugation, sucrose gradient, a commercial kit based on surfactants) on ten different cell lines. Samples were analysed on several instrumental platforms. Mitochondrial pellets were lysed...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-05-30 | MSV000081121 | MassIVE
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