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TranscriptomicsDefl1 knockdown gene expression in Zebrafish 12.5 hpf embryos
Microarray analysis was performed on tail bud stage morphants and stage-matched control injected embryos in order to determine any global gene expression changes occurring as a result of defbl1 knockdown. 2 biological replicates per treatment on single dye Agilent Array
ORGANISM(S): Danio rerio 
2012-01-01 | E-GEOD-31766 | biostudies-arrayexpress
TranscriptomicsChip-chip from Mouse E10.5 anterior and posterior immortalised limb cell lines and limb tissue for H3K27me3 antibody and from Mouse E10.5 anterior and posterior limb tissue for Ring1B antibody.
Anterior-posterior differences in H3K27me3 and Ring1B enrichment over the 5 prime Hoxd genes in E10.5 murine distal forelimbs. Chromatin immunoprecipitation (ChIP) of H3K27me3 together with Ring1B and by ChIP-on-chip analysis demonstrated that over the 5 prime HoxD locus H3K27me3 enrichment is decre...
ORGANISM(S): Mus musculus 
2012-09-18 | E-GEOD-38526 | biostudies-arrayexpress
TranscriptomicsChip-chip from Mouse MEFs cells for H3K36me3, Srsf1, Psip1(A300-847 antibody).
The p52 isoform of Psip1/Ledgf links histone H3K36 methylation and the regulation of alternative splicing. Chromatin immunoprecipitation (ChIP) of Psip1 together with H3K36me3 and Srsf1 and by ChIP-on-chip analysis demonstrated that H3K36me3, Psip1 and SRSF1 enrichment correlates on the gene bodies...
ORGANISM(S): Mus musculus 
2012-01-01 | E-GEOD-31520 | biostudies-arrayexpress
TranscriptomicsChip-chip from Mouse E11.5 distal limb tissue cells for Gabpa, Ets1, Elf1, Etv4, Etv5 antibody
Two different groups of Ets transcription factors differentially act directly at the ZRS to define the spatial expression of Shh in the limb. Chromatin immunoprecipitation (ChIP) of Gabpa, Ets1, Etv4, Etv5 and Elf1 and by ChIP-on-chip analysis demonstrated that with the exception of Elf1 all bind d...
ORGANISM(S): Mus musculus 
2012-02-01 | E-GEOD-33997 | biostudies-arrayexpress
TranscriptomicsChip-chip from WT and Polycomb Component Kock Out Mouse ES cells for H2AZ, H3K27me3, EZH2 and Ring1B.
The essential histone variant H2A.Z localises to both active and silent chromatin sites. In embryonic stem cells (ESCs), H2A.Z is also reported to co-localise with polycomb repressive complex 2 (PRC2) at developmentally silenced genes. The mechanism of H2A.Z targeting is not clear, but a role for th...
ORGANISM(S): Mus musculus 
2012-04-15 | E-GEOD-36999 | biostudies-arrayexpress
TranscriptomicsPsip1 ChIP using A300-847 antibody (Immunoprecipitates mainly p52 isoform of Psip1) Chip-chip from Mouse MEFs cells with H3K36me3, H3K4me3, Psip1 ChIP using A300-847 antibody
The p52 isoform of Psip1/Ledgf links histone H3K36 methylation and the regulation of alternative splicing. Chromatin immunoprecipitation (ChIP) of Psip1 together with H3K36me3 and H3K4me3 and by ChIP-on-chip analysis demonstrated that, Like H3K36me3, Psip1 is enriched on exons of highly expressed ge...
ORGANISM(S): Mus musculus 
2012-04-11 | E-GEOD-28153 | biostudies-arrayexpress
TranscriptomicsComparision of Psip1/p75, Mll and Menin binding on the Hox and non-Hox genes
Menin binding pattern in WT and Psip1 KO MEFs Menin occupancy is studied over Hox genes Menin ChIP from WT and Psip1 KO MEFs ChIP-chip
ORGANISM(S): Mus musculus 
2014-06-27 | E-GEOD-49180 | biostudies-arrayexpress
TranscriptomicsComparision of Psip1/p75 binding and Mll1 binding sites on Hox and non-Hox genes
Psip1/p75 binds to Hox genes and colocalizes with Mll1 and in Psip1 KO MEFs Mll1 occupancy is reduced over Hox genes Psip1/p75 ChIP using A300-848 abtibody (recognises p75 isoform of Psip1) and Mll1 ChIP from WT and Psip1 KO MEFs ChIP-chip
ORGANISM(S): Mus musculus 
2014-06-27 | E-GEOD-49179 | biostudies-arrayexpress
TranscriptomicsComparision of Bmi-1, Ring1B, H3K27me3, Ser2 Pol II, Ser 5 Pol II binding on Hox and non-Hox genes
Bmi-1, Ring1B, H3K27me3, Ser2 Pol II, Ser 5 Pol II binding pattern in WT and Psip1 KO MEFs Menin occupancy is studied over Hox genes and several non-hox genes Bmi-1, Ring1B, H3K27me3, Ser2 Pol II, Ser 5 Pol II ChIPs from WT and Psip1 KO MEFs
ORGANISM(S): Mus musculus 
2014-06-27 | E-GEOD-49181 | biostudies-arrayexpress
TranscriptomicsPsip1/p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2014-06-27 | E-GEOD-49182 | biostudies-arrayexpress
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