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As proof of interdependent interaction between GSAT, GluTR, and GBP, a crosslinking mass spectrometry experiment was conducted with bilateral and trilateral combinations of the three respective recombinant proteins. Apart from the known GBP – GluTR interaction, combined assays for mutual interaction...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
The CHLD and CHLM genes encode proteins involved in tetrapyrrole biosynthesis pathway. It was proposed that intermediates like magnesium protoporphyrin might play a role in retrograde plastid-to-nucleus signaling. In the present work we provide evidence that altered enzymes activity of the tetrapyrr...
ORGANISM(S): Arabidopsis thaliana 
5-aminolevulinic acid (ALA) is the common precursor of all biological synthezised tetrapyrroles. Inhibition of ALA synthesis results in decreased amounts of chlorophylls, heme, siroheme and phytochrome. It was previously shown that 4 out of 5 Arabidopsis mutants uncoupling nuclear gene expression fr...
ORGANISM(S): Arabidopsis thaliana 
Total RNA was extracted from 3-week-old WT and dja6 dja5 seedlings grown on MS medium. RNA sequencing libraries were constructed and subjected to sequencing using an Illumina Hiseq 2500 platform. After a quality control, the gene expression profiling analysis was performed based on the number of tag...
ORGANISM(S): Arabidopsis thaliana 
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