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Quek2014 - Metabolic flux analysis of HEK cell culture using Recon 2 (reduced version of Recon 2) This model is described in the article: Reducing Recon 2 for steady-state flux analysis of HEK cell culture. Quek LE, Dietmair S, Hanscho M, Martínez VS, Borth N, Nielsen LK. J. B...
2015-04-15 | MODEL1504080000 | BioModels
Aim: Transcriptional analysis of the colon of adult Nkx2.2flox/flox;Villin-Cre (Nkx2.2int) mice versus control Methods: 2 cm of the colon (as measured after the caecum) of 6 week old control and mutant mice were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with t...
ORGANISM(S): Mus musculus 
By applying MC EMiNEM (a novel method based on the concept of Nested Effects Models (NEMs) for the retrieval of functional dependencies between proteins that have pleiotropic effects on mRNA transcription) to the expression data from four gene perturbation studies (three of them unpublished) in Sacc...
ORGANISM(S): Saccharomyces cerevisiae 
Aim: Transcriptional analysis of the duodenum of adult Nkx2.2flox/SD;Villin-Cre (SDint) mice versus control Methods: 2 cm of the duodenum (as measured from the stomach) of 6 week old control and mutant mice were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with t...
ORGANISM(S): Mus musculus 
Aim: Transcriptional analysis of the duodenum of adult Nkx2.2flox/TN;Villin-Cre (TNint) mice versus control Methods: 2 cm of the duodenum (as measured from the stomach) of 6 week old control and mutant mice were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with t...
ORGANISM(S): Mus musculus 
In order to assess the transcriptional modifications of dendritic cells treated with Denileukin diftitox (ONTAK), we profiled the whole transcriptome of monocyte-derived dendritic cells (moDC) from blood of healthy volounteers after stimulation with a maturation cocktail with or without the addition...
ORGANISM(S): Homo sapiens 
In this experiment, dendritic cells and monocytes obtained from melanoma patients who underwent immunotherapy were stimulated with a maturation cocktail then either treated or not treated with denlieukin diftitox (DD; ONTAK) and then subjected to transcription profiling to investigate the effects of...
ORGANISM(S): Homo sapiens 
The gene expression of bone marrow cells of mice enriched for Gremlin1 vs control was measured (n=3). It is not known if endogenous adult mesenchymal stem cells (MSCs) exist.Following culture,perisinusoidal mesenchymal cells can clonally recapitulate the skeletal microenvironment, but this fails to ...
ORGANISM(S): Mus musculus 
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