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TranscriptomicsC2C12 Murine Myoblasts over-expressing Eda2r
C2C12 (ATCC, #CRL-1772), a myoblast cell line from the C3H mouse strain, was grown in DMEM (Dulbecco’s Modified Eagle’s Medium, Gibco), supplemented with 10% fetal bovine serum (FBS) (Euroclone, Pero, Italy) and 2 mM L-glutamine (Gibco), and maintained in culture at 37 °C with 5% CO₂, without reachi...
ORGANISM(S): Mus musculus 
2023-09-01 | E-MTAB-13101 | biostudies-arrayexpress
TranscriptomicsModulation of EDA2R/EDA-A2 signalling in Human Myoblasts
Transfection of human myoblasts was performed using Lipofectamine or electroporation. Skeletal muscle myoblasts isolated from a human male donor were transfected using the Lipofectamine™ 2000 Transfection Reagent (Invitrogen, 11668019). Twenty-four hours before transfections, cells were plated to a ...
ORGANISM(S): Homo sapiens 
2024-09-13 | E-MTAB-14239 | biostudies-arrayexpress
TranscriptomicsAll-trans retinoic reduces the levels of cardiolipins in the NB4 acute promyelocytic leukemia cell-line affecting the autophagy and differentiation responses
All-trans retinoic (ATRA) is used in the treatment of acute promyelocytic leukemia a rare form of AML characterized by the presence of a specific translocation involving chrosomes 15;17 and causing the production of the PML-RARα fusion protein in 95% of the cases. ATRA differentiates the APL blasts...
ORGANISM(S): Homo sapiens 
2022-01-31 | E-MTAB-10267 | biostudies-arrayexpress
GenomicsGeneration of corrected hiPSC clones from a Cornelia de Lange Syndrome (CdLS) patient through CRISPR-Cas based technology
Cornelia de Lange syndrome (CdLS) is an autosomal dominant disease mainly caused by mutations in the Nipped-B-like protein (NIPBL) gene resulting in the alteration of the cohesin pathway. Here, we generated human induced pluripotent stem cells (hiPSCs) from a CdLS patient carrying a mutation in the ...
ORGANISM(S): Homo sapiens 
2023-03-17 | E-MTAB-11604 | biostudies-arrayexpress
TranscriptomicsSEPN1-related myopathy depends on the ER oxidase ERO1 alpha and is responsive to the chemical chaperone TUDCA
SEPN1 is a type II protein of the endoplasmic reticulum (ER) whose loss of function gives rise to a collection of debilitating autosomal recessive myopathies gathered under the umbrella term of SEPN1-related myopathy (RM). At the moment, SEPN1-RM lacks an effective pharmacological treatment; thus, t...
ORGANISM(S): Mus musculus 
2024-02-14 | E-MTAB-12460 | biostudies-arrayexpress
TranscriptomicsSmall molecule-mediated ERO1A inhibition in restraining aggressive breast cancer
Cancer cells adapt to harsh environmental conditions by inducing the Unfolded Protein Response (UPR), of which ERO1A is one of the mediators. ERO1A aids protein folding by acting as a protein disulfide oxidase, and under cancer-related hypoxia conditions, it favors the folding of angiogenic VEGFA, l...
ORGANISM(S): Mus musculus 
2025-02-12 | E-MTAB-14269 | biostudies-arrayexpress
TranscriptomicsTreatment with onvansertib, a specific PLK1 inhibitor, overcomes olaparib resistance in high-grade serous ovarian carcinoma patient-derived xenografts
Inhibitors of PARP (PARPis) represent the first clinically approved anticancer agents targeting the DNA damage response. They have been approved as monotherapy and/or in combination and maintenance settings in different tumor types, including high-grade ovarian carcinomas. Their efficacy was first u...
ORGANISM(S): Mus musculus 
2023-09-14 | E-MTAB-13055 | biostudies-arrayexpress
TranscriptomicsMesenchymal stromal cell secretome and its key bioactive metabolites induce long-term neuroprotection after traumatic brain injury in mice
The severe and long-term consequences of traumatic brain injury (TBI) highlight the urgent need for effective neuroprotective therapies. Mesenchymal stromal cells (MSCs) show promise in TBI treatment through their secretome (conditioned media, CM). We previously identified a low-molecular-weight (<7...
ORGANISM(S): Mus musculus 
2025-06-18 | E-MTAB-14942 | biostudies-arrayexpress
TranscriptomicsDOCK1 gene derived hsa_circ_0020397 RNA is over-expressed in luminal mammary tumors and it is involved in the epithelial differentiation, growth and motility of the neoplastic cell
Circular-RNAs are an emerging group of regulatory molecules involved in the control of numerous cellular processes and may play a role in the growth and diffusion of various types of tumors. In the present study, we define the expression of 15 selected circular-RNAs, which may be involved in the pr...
ORGANISM(S): Homo sapiens 
2022-06-15 | E-MTAB-10819 | biostudies-arrayexpress
TranscriptomicsIncreased expression of Ectodysplasin A2 Receptor EDA2R is the most remarkable and ubiquitous aging-related transcriptional hallmark (Murine Myotubes)
We report that increased mRNA-expression of Ectodysplasin-A2-Receptor (EDA2R) is the most remarkable aging-associated transcriptional perturbation in humans and that its induction is confirmed in murine-models of Hutchinson-Gilford progeria syndrome and in other species. Up-regulation of EDA2R occur...
ORGANISM(S): Mus musculus 
2024-09-13 | E-MTAB-14234 | biostudies-arrayexpress
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