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TranscriptomicsSingle-cell RNAseq of control chimeric mouse embryos at embryonic days 7.5 and 8.5 of mouse development
Chimeric mouse embryos were generated as a control experiment to understand the contribution of host vs. injected cells to the developing embryo. Embryos were generated by blastocyst injection of tdTomato-labelled mouse embryonic stem cells into wild type embryos. After blastocyst harvest, cells wer...
ORGANISM(S): Mus musculus 
2018-12-05 | E-MTAB-7324 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNAseq of Tal1 knockout chimeric mouse embryos at embryonic day 8.5 of mouse development
Chimeric embryos were generated to investigate the effect of Tal1 knockout in mouse embryos by single-cell RNA-sequencing. Tal1 is an essential transcription factor for the formation of the embryonic blood. Embryo chimerism permits the analysis of the effects of Tal1 knockout without the confounding...
ORGANISM(S): Mus musculus 
2018-12-05 | E-MTAB-7325 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNAseq of Brachyury knockout chimeric mouse embryos at embryonic days 8.5 and 7.5 of mouse development.
Chimeric embryos were generated to investigate the effect of T knockout in mouse embryos by single-cell RNA-sequencing. T is an essential transcription factor for axial embryonic patterning. Chimeric embryos contain tissue that is T+/+, which prevents global developmental failures. Embryos were gene...
ORGANISM(S): Mus musculus 
2020-08-31 | E-MTAB-8811 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNAseq of Tal1 knockout chimeric mouse embryos at embryonic days 7.5 and 8.5 of mouse development.
Chimeric embryos were generated to investigate the effect of Tal1 knockout in mouse embryos by single-cell RNA-sequencing. Tal1 is an essential transcription factor for the formation of the embryonic blood. Embryo chimerism permits the analysis of the effects of Tal1 knockout without the confounding...
ORGANISM(S): Mus musculus 
2018-12-05 | E-MTAB-6968 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNAseq of additional control chimeric mouse embryos at embryonic day 8.5 of mouse development
This dataset contains additional data performed as in the experiment stored at accession E-MTAB-7324. Chimeric mouse embryos were generated as a control experiment to understand the contribution of host vs. injected cells to the developing embryo. Embryos were generated by blastocyst injection of t...
ORGANISM(S): Mus musculus 
2020-08-31 | E-MTAB-8812 | biostudies-arrayexpress
TranscriptomicsTracking Early Mammalian Organogenesis – Prediction and Validation of Differentiation Trajectories at Whole Organism Scale
This project is based on the extension of the single cell transcriptomics atlas of mouse development towards E9.5. Inference of cell differentiation trajectories was carried out using Waddington-Optimal Transport, and the resulting computational reconstruction was contrasted with grafting experiment...
ORGANISM(S): Mus musculus 
2023-01-01 | E-MTAB-11763 | biostudies-arrayexpress
TranscriptomicsTimecourse single-cell ATACseq of whole rabbit embryos sampled across gestational days 7, 8 and 9
We characterised the chromatin accessibility profiles of 34,082 individual cells (post-QC) from whole rabbit embryos spanning gestational days 7, 8 and 9. Cell types were labelled via label transfer of simultaneously generated scRNA-seq of whole rabbit embryos at the same stages. This data was used ...
ORGANISM(S): Oryctolagus cuniculus 
2022-11-30 | E-MTAB-11804 | biostudies-arrayexpress
TranscriptomicsTimecourse single-cell RNA-seq of whole rabbit embryos sampled across gestational days 7, 8 and 9
We characterised the transcriptomic profiles of 146,133 individual cells (post-QC) from whole rabbit embryos spanning gestational days 7, 8 and 9. These experiments were performed to elucidate the molecular programmes underlying gastrulation and early organogenesis in a non-rodent mammal. Combined w...
ORGANISM(S): Oryctolagus cuniculus 
2022-08-26 | E-MTAB-11836 | biostudies-arrayexpress
TranscriptomicsTimecourse single-cell RNAseq of whole mouse embryos harvested between days 6.5 and 8.5 of development.
We have captured 100,000 single cells for single-cell RNAseq from whole mouse embryos during gastrulation and organogenesis, spanning days 6.5 to 8.5 of development, including embryonic and extraembryonic tissues. Cells were sampled every six hours, providing a continuous molecular characterisation ...
ORGANISM(S): Mus musculus 
2018-12-05 | E-MTAB-6967 | biostudies-arrayexpress
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