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GenomicsChIP-seq of Klf2 in mouse embryonic stem cells (Serum/LIF and 2i)
The self-renewing pluripotent state was first captured in mouse embryonic stem cells (mESCs) over two decades ago. The standard condition requires the presence of serum and LIF, which provide growth promoting signals for cell expansion. However, there are pro-differentiation signals which destabiliz...
ORGANISM(S): Mus musculus 
2014-06-04 | E-MTAB-2365 | biostudies-arrayexpress
ProteomicsMYC2 phosphorylation
Mass spectrometry identification of MYC2 phosphorylation by the kinase Feronia
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2017-04-20 | MSV000080972 | MassIVE
ProteomicsArabidopsis thaliana whole-proteome BIN2 kinase assay
Finely ground leaf tissue of wild-type columbia-0 ecotype was used. Protein extraction was performed in buffer-saturated phenol and high sucrose buffer. Extracted proteins were incubated with purified recombinant GST or GST-BIN2 kinase. Filter-assisted sample preparation (FASP) columns in prese...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086462 | MassIVE
ProteomicsBIN2 and RAPTOR1B mutants phosphoproteomic analysis
Finely ground leaf tissue was used. Extraction was done in 8M urea, precipitated with acetone and methanol. Filter-assisted sample preparation (FASP) columns were used to further purify proteins. Purified proteins were reduced with TCEP, alkylated with iodoacetamide and digested into peptides with ...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086460 | MassIVE
TranscriptomicsTranscriptional profiling by array of Klf2 wild-type and Klf2-Null mESCs cultured under 2i
The self-renewing pluripotent state was first captured in mouse embryonic stem cells (mESCs) over two decades ago. The standard condition requires the presence of serum and LIF, which provide growth promoting signals for cell expansion. However, there are pro-differentiation signals which destabiliz...
ORGANISM(S): Mus musculus 
2014-06-04 | E-MTAB-2366 | biostudies-arrayexpress
ProteomicsProtein abundance and phosphorylation changes in feronia mutant arabidopsis rosette leaves
The goal of this study was to determine protein abundance and phosphorylation changes in the FERONIA mutant (fer) in Arabidopsis leaves. Seeds were germinated on 1/2MS with constant light (24hr light) for 10 days. Then, plants were transferred to soil and grown under short day condition (8-hour ligh...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-01-14 | MSV000084804 | MassIVE
ProteomicsUbiquitylome analysis of Arabidopsis roots.
Ubiquitylome analysis of Arabidopsis roots. 10 day old Arabidopsis roots were treated with mock, DMSO, or bortezomib for 3 hours. Root tissue was processed using the phenol-FASP method described in https://doi.org/10.1002/pmic.201800220. Ubiquitin remnant peptides were enriched using an anti-diglyci...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
MSV000086156 | MassIVE
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