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TranscriptomicsIdentification of protein-RNA interactions in Drosophila using RIP-Seq
The goal of these experiments is to identify RNAs bound by individual RNA-binding proteins. Drosophila S2R+ cells were transiently transfected with vectors encoding the indicated HA-tagged RNA-binding proteins, or without an insert as a control. Expression was induced with CuSO4, whole cell lysates ...
ORGANISM(S): Drosophila melanogaster 
2012-05-05 | E-GEOD-37756 | biostudies-arrayexpress
UnknownNext-generation transcriptome sequencing of the premenopausal breast epithelium using specimens from a normal human breast tissue bank.
Not available
S-EPMC4053088 | biostudies-literature
TranscriptomicssiRNA profiling of human steroid-deprived LNCaP cells were transfected with control or ART-27 siRNA to investigate the genome-wide impact of ART-27 loss on androgen-regulated transcription
The androgen receptor (AR) directs diverse biological processes through interaction with coregulators such as androgen receptor trapped clone-27 (ART-27). The impact of ART-27 on genome-wide transcription was examined. The studies indicate that loss of ART-27 enhances expression of many androgen-reg...
ORGANISM(S): Homo sapiens 
2009-06-08 | E-GEOD-14043 | biostudies-arrayexpress
UnknownCharacterizing the heterogeneity of triple-negative breast cancers using microdissected normal ductal epithelium and RNA-sequencing.
Not available
S-EPMC3901081 | biostudies-literature
ProteomicsMudPIT analyses of the proteins co-purified with Ada2b-PB-FLAG-HA FLAG-affinity purified from Drosophila melanogaster S2 cells
Protein Complexes Purification- Stable S2 cell lines expressing Ada2b (isoform B; NP_001027151.1) in the pRmHa3-CHA2FL2 (Ada2b-PBH2F2) were maintained at 1-2 e6 cells/mL in SFX medium (HyQ SFX-Insect). Cells stably transfected with empty vector served as negative controls. Protein complexes containi...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2018-11-20 | MSV000083153 | MassIVE
TranscriptomicsImpacts of Salmonella enteritidis Infection on Liver Transcriptome in Broilers
To provide insight into the systemic metabolic effects of S. enteritidis infection, liver samples were harvested 10 days post infection from broiler hens. Hepatic global gene expression levels were assessed using a chicken 44K Agilent microarray. Forty-four genes were differentially expressed at a s...
ORGANISM(S): Gallus gallus 
2013-02-17 | E-GEOD-39448 | biostudies-arrayexpress
TranscriptomicsNRASG12V mediates self renewal in AML [RNA-Seq]
Mutant RAS oncoproteins activate signaling molecules that drive oncogenesis in multiple human tumors including acute myelogenous leukemia (AML). However, the specific function of these pathways in AML is unclear. To elucidate the downstream functions of activated NRAS in AML, we employed a murine mo...
ORGANISM(S): Mus musculus 
2014-11-16 | E-GEOD-49087 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of vastus lateralis muscle biopsies from young and old male human subjects
This series includes the global gene expression profile of the vastus lateralis muscle for 10 young (19-25 years old) and 12 older (70-80 years old) male subjects.
ORGANISM(S): Homo sapiens 
2007-08-04 | E-GEOD-1428 | biostudies-arrayexpress
UnknownThe Immune Microenvironment in Hormone Receptor-Positive Breast Cancer Before and After Preoperative Chemotherapy.
Not available
S-EPMC6677598 | biostudies-literature
ProteomicsA pipeline for discovery and verification of glycoproteins from plasma-derived extracellular vesicles as breast cancer biomarkers
Glycoproteins comprise more than half of current FDA-approved protein cancer markers but the development of new glycoproteins as disease biomarkers has been stagnant. Here we present a pipeline to develop glycoproteins from extracellular vesicles (EVs) through integrating quantitative glycoproteomic...
ORGANISM(S): Homo sapiens (Human) 
2018-04-26 | PXD007572 | Pride
Extracellular vesicles Glycoproteomics Biomarker 
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