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TranscriptomicsTranscription profiling of human lymphatic endothelial cells, mouse dermal cells and human skin cells reveals an inflammation-induced mechanism for leukocyte transmigration of lymphatic vessel endothelium.
The exit of antigen-presenting cells (APC) and lymphocytes from inflamed skin to afferent lymph is vital for the initiation and maintenance of dermal immune responses. How such exit is achieved and how cells transmigrate the distinct endothelium of lymphatic vessels is however unknown. Here we show ...
ORGANISM(S): Homo sapiens 
2008-11-07 | E-GEOD-6257 | biostudies-arrayexpress
ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from human
Mammalian protein extraction and Halo purification For Halo purification, HEK293T cells at 40% confluency in a 100 mm plate were transfected with 10ug of plasmid containing ORF of PDHA1-HA-Halo in 1X BBS with 125 mM CaCl2. Cells were harvested by scraping in PBS following PBS wash after 24 hours fro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-03-20 | MSV000085125 | MassIVE
ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from Saccharomyces cerevisiae by FLAG-affinity purification and Size Exclusion Chromatography
Yeast protein extractions and subcellular fractionation Yeast cells expressing FLAG tagged proteins were grown in YPD medium at 30oC to OD600 of 0.8. 12 Liters YPD culture of cells were pelleted and washed in SB buffer. The pellet was resuspended in SB buffer and treated with 2 mg/ml Zymolyase 20T ...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-03-20 | MSV000085126 | MassIVE
TranscriptomicsModulation of murine bone marrow derived dendritic cells by IL-4
The archetypal Th2 cytokine interleukin 4 (IL-4) has previously been shown to alternatively activate dendritic cells (DCs) both in vitro and in vivo.To more fully understand the impact of IL-4 dependent alternative activation of DCs, we used mRNA microarrays to define their transcriptional profile. ...
ORGANISM(S): Mus musculus 
2015-04-28 | E-GEOD-59868 | biostudies-arrayexpress
ProteomicsProteogenomic characterization of human colon and rectal cancer
Explore This Study at the NCI Proteomic Data Commons

Zhang, B., et al., Nature (2014) do...

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-08-26 | MSV000084238 | MassIVE
GenomicsAtlas of the chromatin landscape in the developing nervous system of Drosophila melanogaster - ChIPseq and ATACseq - time series of tissue specific neuroblast histone PTMs
In this study, I identify novel regulatory nodes and potential signal transduction pathways that govern neurogenic tissue specification and differentiation. The Drosophila melanogaster central nervous system develops from three columns of neuroectodermal cells that give rise to neural stem cells cal...
ORGANISM(S): Drosophila melanogaster 
2025-10-30 | E-MTAB-15767 | biostudies-arrayexpress
TranscriptomicsSelective mRNA translation during eIF2 phosphorylation induces expression of IBTKalpha
Disruption of protein folding in the endoplasmic reticulum triggers the Unfolded Protein Response (UPR), a transcriptional and translational control network designed to restore protein homeostasis. Central to the UPR is PERK phosphorylation of the alpha subunit of eIF2 (eIF2~P), which represses glob...
ORGANISM(S): Mus musculus 
2014-06-02 | E-GEOD-54581 | biostudies-arrayexpress
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