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TranscriptomicsTranscription profiling of mouse preimplantation development: unfertilized eggs, fertilized egg, 2-cell embryos, 4-cell embryos, 8-cell embryos, morulae, blastocysts
Feature Extraction 5.1.1 software (Agilent Technologies) performs background subtractions and dye normalization. This normalization method is targeted at detecting changes in relative expression of individual genes rather than global expression. Global expression change would require external normal...
ORGANISM(S): Mus musculus 
2004-01-12 | E-MEXP-74 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice
Decreasing oocyte competence with maternal aging is a major factor in human infertility. To investigate the age-dependent molecular changes in a mouse model, we compared the expression profiles of metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice usi...
ORGANISM(S): Mus musculus 
2004-09-01 | E-MEXP-150 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of global gene expression changes during mouse preimplantation development: unfertilized eggs, fertilized egg, 2-cell embryos, 4-cell embryos, 8-cell embryos, morulae, blastocysts
Microarray data analysis Intensity of 21,939 gene features per array was extracted from scanned microarray images using Feature Extraction 5.1.1 software (Agilent Technologies), which performs background subtractions and dye normalization. This normalization method is targeted at detecting changes i...
ORGANISM(S): Mus musculus 
2004-01-30 | E-MEXP-73 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryos and placentas pooled within 3 litters to validate a new array
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used t...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-69 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E12.5 mouse whole embryos and placentas pooled within 3 litters and used to valdiate a new array
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was u...
ORGANISM(S): Mus musculus 
2005-01-01 | E-MNIA-68 | biostudies-arrayexpress
TranscriptomicsAge-associated alteration of gene expression patterns in mouse oocytes
Decreasing oocyte competence with maternal aging is a major factor in human infertility. To investigate the age-dependent molecular changes in a mouse model, we compared the expression profiles of metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice us...
ORGANISM(S): Mus musculus 
2010-06-06 | E-GEOD-1646 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part2
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was u...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3513 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part 3
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples at various input levels. A "full-scale" reference was prepared using the standard input level of 6.0 ug total RN...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3507 | biostudies-arrayexpress
TranscriptomicsIn situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part1
E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used t...
ORGANISM(S): Mus musculus 
2010-06-30 | E-GEOD-3516 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of parthenogenetic mouse embryos treated with cycloheximide or strontium chloride
gene expression pattern of parthenogenetic mouse embryos by cycloheximide or Sr2+.
ORGANISM(S): Mus musculus 
2006-06-05 | E-MNIA-85 | biostudies-arrayexpress
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