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TranscriptomicsAIN-2::GFP IP of mRNAs associated with miRISC in stage sychronized C. elegans populations
AIN-2::GFP IP were used to purify miRISC from stage sychronized C.elegans populations (Egg, L1, L2, L3 and L4 stages). The mRNA composition of the IP results and the corresponding total RNA samples were analyzed by WUSTL Caenorhabditis elegans Whole Genome 23k Oligo Array. The miRISC associated mRNA...
ORGANISM(S): Caenorhabditis elegans 
2009-05-21 | E-GEOD-16208 | biostudies-arrayexpress
GenomicsMultiple roles for Piwi in silencing Drosophila transposons
Silencing of transposons in the Drosophila ovary relies on three Piwi-family proteins, Piwi, Aubergine (Aub), and Ago3, acting in concert with their small RNA guides, the piRNAs. Aub and Ago3 are found in the germ cell cytoplasm, where they function in the ping-pong cycle to consume transposon mRNA...
ORGANISM(S): Drosophila melanogaster 
2013-02-09 | E-GEOD-43360 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of leafbladeless1-regulated and phased small RNAs provides new insights into the tas3 pathway in maize-
Maize LEAFBLADELESS1 (LBL1) and Arabidopsis SUPPRESSOR OF GENE SILENCING3 (SGS3) play orthologous roles in the biogenesis of 21 nucleotide trans-acting short-interfering RNAs (tasiRNAs). The phenotypes conditioned by mutation of lbl1 and SGS3 are, however, strikingly different, suggesting that the a...
ORGANISM(S): Zea mays 
2014-11-20 | E-GEOD-50557 | biostudies-arrayexpress
TranscriptomicsRNF17 referees ping-pong in mouse testes [5'RACE]
In mammals, piRNA populations are dynamic throughout male germ cell development. Embryonic piRNAs consist of both primary and secondary species and are mainly directed toward transposons. In meiotic cells, however, the piRNA population is transposon-poor and restricted to primary piRNAs derived from...
ORGANISM(S): Mus musculus 
2014-12-17 | E-GEOD-53915 | biostudies-arrayexpress
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