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TranscriptomicsSmall non-coding RNA expression in murine germ cells
In order to monitor the changes in small RNAs expression during mouse spermatogenesis. Type A spermatogonia, pachytene spermatocytes and round spermatids were isolated following collagenase treatment of testes and trypsin digestion of isolated seminiferous tubules using unit gravity sedimentation in...
ORGANISM(S): Mus musculus 
2011-06-10 | E-GEOD-24822 | biostudies-arrayexpress
TranscriptomicsRetinoic Acid Is Sufficient for the in vitro Induction of Mouse Spermatocytes
To identify RA-regulated genes in mSSCs, we treated mSSCs with vehicle, RA and RA plus CHX. To characterize the induced spermatogenic cells by transcriptome profiling, we cultured mSSCs on pup Sertolic cells supplemented with RA. Induced differentiating spermatogonia and induced spermatocytes were c...
ORGANISM(S): Mus musculus 
2016-06-16 | E-GEOD-77051 | biostudies-arrayexpress
TranscriptomicsEpigenetic dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis
Studies on the epigenetic regulation of gene expression during germ cell development are still at the beginning stage. In the present study, we used our highly specific 5hmC-labeling and enrichment technique coupled with DNA deep-sequencing to profile the global 5hmC distribution in 8 serial stages ...
ORGANISM(S): Mus musculus 
2012-03-12 | E-GEOD-35005 | biostudies-arrayexpress
TranscriptomicsFGF2-regulated gene expression in cultured mouse spermatogonial stem cells
Transcriptional profiling of mouse spermatogonial stem cells (SSCs) comparing control untreated SSCs with SSCs with exogenous FGF2 withdrawn and FGFR inhibitor SU5402 supplemented (-F+S). Results provide insight into the mechanisms of FGF2-supported in vitro self-renewal of SSCs. Two-condition exper...
ORGANISM(S): Mus musculus 
2010-08-01 | E-GEOD-21294 | biostudies-arrayexpress
Transcriptomicssmall RNA deep-sequencing of mature sperm, testis and uterus in mice
In order to discover novel small RNAs expressed in mature sperm, we isolated mature sperm from mouse cauda epididymis, comparing with data from adult tesis and uterus. The small RNA fraction (18-40nt) was cloned and sequenced from total RNA of mature sperm, testis and uterus of mice. RNAs extracted ...
ORGANISM(S): Mus musculus 
2013-01-31 | E-GEOD-38702 | biostudies-arrayexpress
TranscriptomicsSmall non-coding RNA expression in mouse elongated spermatids
In order to discover novel small RNAs expressed in elongated spermatids, we isolated elongated spermatids from mouse testis. The small RNA fraction (18-40nt) was cloned and sequenced from total RNA. RNAs extracted from elongated spermatids were used for high throughput sequencing analysis
ORGANISM(S): Mus musculus 
2013-01-31 | E-GEOD-40397 | biostudies-arrayexpress
TranscriptomicsAdult_mouse_testis_CREM_ChIP_chip
Germ cells from adult mouse testes were isolated and ChIPed by CREM antibody. The purpose is to identify potential CREM targets during spermatogenesis. The CREM antibody ChIPed DNA segments were labeled with Cy5 and goat IgG ChIPed segments were labeled with Cy3 as control. No duplicates were design...
ORGANISM(S): Mus musculus 
2010-10-13 | E-GEOD-24623 | biostudies-arrayexpress
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