In this study, we carried out genome-scale CRISPRi modulation combining fluorescence-activated cell sorting (FACS) and next-generation sequencing (NGS) to identify genetic determinants for FFAs overproduction in Escherichia coli. The pcnBi (AP) strain that repressed the expression of pcnB produced 2...
This file include 9 sample: heart sample from WT mice (1, 2, 3)+heart sample from WT mice treated with DOX (4,5,6)+heart sample from HINT2 cKO mice treated with DOX (7,8,9))
In this project, SILAC-based quantitative proteomics approach was applied for phosphoproteomics analysis. The light samples were treated with ddH2O as control group, and the medium samples labeled by 2D2 of lysine (K4) and 13C6 of arginine (R6) were treated with 2DG, while the heavy samples labeled ...
