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MetabolomicsPhenotypes and Multi—omics Reveal Changes and Molecular Mechanism of Suspension Adaptation of HEK293 Cells Structural Remodelling, Metabolic Reconstruction and Stress Resistance

Human embryonic kidney 293 (HEK293) cells have been successfully adapted from adherent to suspension culture and have been widely applied in both scientific research and the pharmaceutical industry. However, the alterations in cells during the adaptation have not been well described, which raise ...

2025-12-16 | MTBLS13517 | MetaboLights
MetabolomicsMetabolic Response of HEK 293 Cells Exposed to Methylmercury
Not available
ORGANISM(S): Homo sapiens 
ST001776 | MetabolomicsWorkbench
TranscriptomicsTranscriptome of HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1)
Comparison of gene expression profile of HEK293-CT cells and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1) We used micrarrays to identify the repertoire of genes differentially expressed upon overexpression of the chromatin repressor BAHD1 in HEK293 cells.
ORGANISM(S): Homo sapiens 
2015-12-11 | E-GEOD-51863 | biostudies-arrayexpress
GenomicsMethylated DNA immunoprecipitation-sequencing of HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1)
we report the partial methylome (CG-rich regions) of HEK293 cells and HEK293 cells over-expressing the BAHD1 gene (HEK-BAHD1) We used MEDIP-seq to identify genomic regions differentially methylated upon overexpression of the chromatin repressor BAHD1 in HEK293 cells.
ORGANISM(S): Homo sapiens 
2015-12-11 | E-GEOD-51864 | biostudies-arrayexpress
Genomicswhole-genome bisulfite sequencing (BS-seq) of HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1)
Comparison of methylome of HEK293-CT cells and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1) We used BS-seq to identify genomic regions differentially methylated upon overexpression of the chromatin repressor BAHD1 in HEK293 cells.
ORGANISM(S): Homo sapiens 
2015-12-11 | E-GEOD-51867 | biostudies-arrayexpress
TranscriptomicsRole of BACH1 in HEK 293T cells
This SuperSeries is composed of the following subset Series: GSE28050: Expression data from knockdown of BACH1 in HEK 293T cells GSE28051: Genome-wide map of BACH1 binding in HEK293T cells Refer to individual Series
ORGANISM(S): Homo sapiens 
2011-05-12 | E-GEOD-28053 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human monoclonal cell lines with high or low levels of store-operated Ca2+ entry
This series of microarray experiments monitored the gene expression profiles for monoclonal cell lines (derived from HEK-293 parental cell culture) with high (H1, H15, H24, H36, H39) or low (L3, L28, L29) levels of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded ...
ORGANISM(S): Homo sapiens 
2007-08-03 | E-GEOD-1309 | biostudies-arrayexpress
ProteomicsExome-based proteogenomics of HEK-293 human cell line: coding genomic variants identified at the level of shotgun proteome
Genomic and proteomic data were integrated into the proteogenomic workflow to identify coding genomic variants of Human Embryonic Kidney 293 (HEK-293) standard cell line at the proteome level. Shotgun proteome data published by Geiger et al (2012) and obtained in this work for HEK-293 were searched ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-28 | MSV000080712 | MassIVE
MetabolomicsLipids in the escalated host-pathogen arms race

Lipids, fundamental constituents of cell membranes, cellular energy carriers, and signaling molecules, play a pivotal role in the interaction between pathogens and hosts. During microbial infections, lipids not only fuel the activation of the immune system but also, acting as signaling molec...

2025-07-05 | MTBLS12678 | MetaboLights
TranscriptomicsMIR-204 target genes
The aim of this study is to discover genes regulated by miR-204. Differential gene expression in HEK-293 cells transfected with miR-204-mimic compared to HEK-293 cells transfected with control oligo (HEK-293 control) was analyzed using the Agilent Human Whole Genome 4x44K gene expression array (Agil...
ORGANISM(S): Homo sapiens 
2012-03-30 | E-GEOD-28400 | biostudies-arrayexpress
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