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ProteomicsEvaluation of volumetric absorptive microsampling and mass spectrometry data-independent acquisition in hemoglobin related clinical markers
Data-independent acquisition (DIA) allows comprehensive proteome coverage, and it has the potential to work as a unified protocol to determine a multitude of proteins in blood. Because of its high specificity, mass spectrometry has a high potential to reduce the interferences presented by other assa...
ORGANISM(S): Homo Sapiens 
2022-09-25 | PXD029918 | panorama
TranscriptomicsEHMT1 and EHMT2 inhibition induce fetal hemoglobin expression [RNA-seq]
Using UNC0638 and genetic assays to inhibit EHMT1/2 and derepress fetal hemoglobin in adult hematopoietic cells. RNA-Seq in primary adult human erythroid cells treated with UNC0638 or the vehicle control (DMSO) in biological triplicates.
ORGANISM(S): Homo sapiens 
2015-11-02 | E-GEOD-71421 | biostudies-arrayexpress
GenomicsEHMT1 and EHMT2 inhibition induce fetal hemoglobin expression [ChIP-seq]
Using UNC0638 and genetic assays to inhibit EHMT1/2 and derepress fetal hemoglobin in adult hematopoietic cells. ChIP-seq for 2 histone modifications and total H3 (for quantitative normalization) in biological triplicates of cord blood, adult bone marrow, and UNC0638-treated adult bone marrow.
ORGANISM(S): Homo sapiens 
2015-11-02 | E-GEOD-69516 | biostudies-arrayexpress
TranscriptomicsmiRNA expression profiles of erythroid cells following hydroxyurea treatment for sickle cell anemia
We hypothesized that miRNA regulation may be invloved in hydroxyurea-mediated fetal hemoglobin induction. Microarray analysis was utilized as an initial screening tool to determine differential miRNA expression in CD71+ erythroid cells comparing cells from control individuals without sickle cell ane...
ORGANISM(S): Homo sapiens 
2011-11-21 | E-GEOD-32035 | biostudies-arrayexpress
ProteomicsHydroxyl radical footprinting analysis of a human haptoglobin-hemoglobin complex
Methods of structural mass spectrometry have become more popular to study protein structure and dynamics. Among them, fast photochemical oxidation of proteins (FPOP) has several advantages such as irreversibility of modifications and more facile determination of the site of modification with single ...
ORGANISM(S): Homo sapiens (Human) 
2021-12-30 | PXD021621 | Pride
Footprinting Hemoglobin Haptoglobin Fpop Timstof pro 
ProteomicsHb Oxidation in stored RBCs and Vesicles
We used a state of the art proteomics workflow, based upon nano-UPLC-MS\MS and advanced database searching to identify oxidative modifications to functional residues of hemoglobin subunit beta . Progressive accumulation of oxidized residues in stored erythrocytes and selective removal in vesicles wa...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-04 | MSV000080222 | MassIVE
TranscriptomicsPrimary human erythroid progenitor cells SAHA treatment samples
Gene expression profiling was performed on primary human erythroid progenitor cells left untreated or treated with 0.5uM SAHA. The worldwide burden of sickle cell disease is enormous, with over 200,000 infants born with the disease each year in Africa alone. Induction of fetal hemoglobin is a valida...
ORGANISM(S): Homo sapiens 
2010-07-29 | E-GEOD-22367 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human vascular smooth muscle cells treated with glucose oxidase and human haemoglogib reveals ferrous hemoglobin can protect vascular smooth muscle cells from hydrogen peroxide-induced oxidative stress response and cell damage
Hemoglobin (Hb) is released from red blood cells (RBC) during intravascular hemolysis. Cell free Hb has been implicated to play a pathogenic role in hemolytic diseases such as sickle cell anemia or malaria. Hydrogen peroxide (H2O2) is the most prevalent reactive oxygen species which is produced in e...
ORGANISM(S): Homo sapiens 
2009-09-15 | E-MEXP-2263 | biostudies-arrayexpress
TranscriptomicsPrimary human erythroid progenitor cells HDAC1 and HDAC2 shRNA knockdown samples
Gene expression profiling was performed on primary human erythroid progenitor cells expressing a control shRNA (luciferase), two different HDAC1 shRNAs, and two different HDAC2 shRNAs. The worldwide burden of sickle cell disease is enormous, with over 200,000 infants born with the disease each year ...
ORGANISM(S): Homo sapiens 
2010-07-29 | E-GEOD-22366 | biostudies-arrayexpress
TranscriptomicsRNAi profiling by array of human CD34-derived erythroid progenitors treated with BCL11A siRNAs
Differences in the amount of fetal hemoglobin (HbF) that persists into adulthood affect the severity of sickle cell disease and the beta-thalassemia syndromes. Genetic association studies have identified sequence variants in the gene BCL11A that influence HbF levels. Here we examine BCL11A as a pote...
ORGANISM(S): Homo sapiens 
2009-01-13 | E-GEOD-13284 | biostudies-arrayexpress
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