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TranscriptomicsIdentifying crossover locations in an Arabidopsis thaliana Col x Ler F2 population using genotyping by sequencing.
Genomic DNA from 192 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input for each library. ...
ORGANISM(S): Arabidopsis thaliana 
2016-06-11 | E-MTAB-4657 | biostudies-arrayexpress
TranscriptomicsIdentifying crossover locations in an Arabidopsis thaliana HEI10 Col x Ler F2 population using genotyping by sequencing.
Genomic DNA from 192 HEI10 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input for each lib...
ORGANISM(S): Arabidopsis thaliana 
2016-08-05 | E-MTAB-4967 | biostudies-arrayexpress
TranscriptomicsIdentifying crossover locations in Arabidopsis thaliana wild type and taf4b Col × Bur F2 populations using genotyping-by-sequencing
Genomic DNA from 180 Col/Bur F2 individuals, and 186 taf4b/Bur F2 individuals, was extracted by CTAB and used to generate sequencing libraries as previously described (Ziolkowski et al, 2017 Genes & Dev). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously desc...
ORGANISM(S): Arabidopsis thaliana 
2019-12-31 | E-MTAB-8099 | biostudies-arrayexpress
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