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TranscriptomicsIdentification of mRNAs Recruited to Argonaute 2 by Specific microRNAs and Corresponding Changes in Transcript Abundance
This SuperSeries is composed of the following subset Series: GSE11079: mRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected GSE11080: Expression data HEK293T cells transfected with either Ago2, Ago2 + miR-1, Ago2 + miR-124, mock GSE11081: miRNA arrays: Ago2 transfected vs. ...
ORGANISM(S): Homo sapiens 
2008-04-07 | E-GEOD-11082 | biostudies-arrayexpress
TranscriptomicsExpression data HEK293T cells transfected with either Ago2, Ago2 + miR-1, Ago2 + miR-124, mock
HEK293T cell expression data from four conditions: mock transfection, Ago2 transfection, Ago2 & miR-1 transfection, and Ago2 & miR-124 transfection. RNA was isolated directly from 10cm dishes using Trizol reagent and amplified. Set of arrays that are part of repeated experiments Compound Based Treat...
ORGANISM(S): Homo sapiens 
2008-04-07 | E-GEOD-11080 | biostudies-arrayexpress
TranscriptomicsmRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected
As a core RISC component, Ago2 associates with miRNAs and target mRNAs. To identify these mRNAs, we ran lysate from HEK293T cells over a FLAG resin from 2 conditions: +FLAG-Ago2, +mock transfection. To identify mRNAs associated with specific miRNAs, we ran lysate from HEK293T cells over a FLAG resi...
ORGANISM(S): Homo sapiens 
2008-04-08 | E-GEOD-11079 | biostudies-arrayexpress
TranscriptomicsmiRNA arrays: Ago2 transfected vs. Mock, Ago2 IP vs. total RNA
miRNAs were enriched from HEK293T cells using the ambion FLASHPAGE fractionater after mock transfection, Ago2 transfection, and FLAG-Ago2 IP. miRNAs were labeled and hybridized: Ago2 transfected vs. mock transfection and Ago2 transfected vs. Ago2 IPs. Set of arrays that are part of repeated experime...
ORGANISM(S): Homo sapiens 
2008-04-07 | E-GEOD-11081 | biostudies-arrayexpress
TranscriptomicsRBP40_arrays
In this study, we systematically identified the RNAs associated with a selective sample of 40 of ~600 yeast RNA-binding proteins (RBPs). To identify RNAs associated with each putative RBP, C-terminal tandem affinity purification (TAP)-tagged proteins, expressed under control of their native promoter...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-25 | E-GEOD-13135 | biostudies-arrayexpress
TranscriptomicsHuman Argonaute Immunoaffinity Purification-associated RNA
Immunoaffinity Purifications of Human Argonaute Twelve hours after transfections with mock or with miR-124, we washed each 15-cm plate once with phosphate-buffered saline (usually two plates were used per IP), then added 1 ml of 4 C lysis buffer (150 mM KCl, 25 mM Tris-HCl [pH 7.4], 5 mM Na-EDTA [p...
ORGANISM(S): Homo sapiens 
2010-06-20 | E-GEOD-18834 | biostudies-arrayexpress
TranscriptomicsTranslation Profiling +/- miR-124
Ribosome Occupancy Experiments: for these experiments Channel 1 is amplified RNA (aRNA) from mRNA unbound to any ribosomes. Channel 2 is aRNA from mRNA associated with ribosomes. Ribosome Density Experiments: for these experiments Channel 1 is amplified RNA (aRNA) from mRNA present in the light fra...
ORGANISM(S): Homo sapiens 
2010-06-20 | E-GEOD-18836 | biostudies-arrayexpress
TranscriptomicsmRNA abundance in HEK293T cells mock or miR-124 transfected
HEK293T cells were either mock or miR-124 transfected. Cells were lysed 12 hours after transfection with Trizol reagent. RNA was isolated, amplified, labeled (cy5), and comparatively hybridized with a universal reference (cy3) on HEEBO arrays in biological triplicate. In addition, each biological re...
ORGANISM(S): Homo sapiens 
2010-06-20 | E-GEOD-18835 | biostudies-arrayexpress
TranscriptomicsUnamplified mRNA abundance in miR-124 and mock-transfected cells
To compare total RNA levels in miR-124 and mock-transfected cells (Figure S3), 5-10 ug of total RNA from miR-124-transfected cells or mock-transfected cells or universal reference RNA (Stratagene Cat# 740000) was reverse transcribed with Superscript III (Invitrogen Cat# 18080085) in the presence of ...
ORGANISM(S): Homo sapiens 
2010-06-20 | E-GEOD-18837 | biostudies-arrayexpress
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